From Shangganling to Crossing the Yalu River: Changes and Invariance in the Inter-generational Change of Films and TV Plays to Resist US Aggression and Aid Korea

The TV series "Meritorious" unit "Capable of Literature and Armed Forces in Li Yannian" regains the theme of resisting US aggression and aiding Korea, and excavates the shining human brilliance in the people’s army in detail.
Sun Xinqi
A few days ago, the film "Crossing the Yalu River" officially landed in major theaters across the country. Based on the TV series of the same name premiered at the end of last year, the film version of Crossing the Yalu River condensed the volume of 40 episodes into two and a half hours, and the depth, breadth and density of the content were highly praised by the industry and outside.
From the first film "Shangganling" released in 1956, to the first TV series "The 38th parallel" premiered in 2016, and then to the panoramic work "Crossing the Yalu River" which was broadcast and released one after another last year and this year, the film and television dramas with the theme of resisting US aggression and aiding Korea experienced a creative peak and once faded out of public view, and now they are welcoming another peak. The past 65 years have witnessed the changes and development of China’s film and television drama industry, and witnessed the enduring theme of resisting US aggression and aiding Korea, and even the spirit of defending the country and defending the country in the creation of film and television dramas.
Narrative angle:
From the grassroots to the top
From what angle to cut into the narrative is the first multiple-choice question that war writers have to answer well. In the early preparation of Shangganling, the film crew considered the panoramic display of this epic battle, but they finally chose to break through the tradition and focus on a tunnel and a company in Shangganling battle, so as to deepen the viewing experience and highlight the fearless spirit of the people’s heroes. Since then, a series of movies on the theme of resisting U.S. aggression and aiding Korea have adopted a similar approach of "seeing the big from the small", and created heroic images that are deeply rooted in people’s hearts, such as Wang Cheng in Heroes’ Children, Fang Yong in Surprise Attack, Zhang lei in Flying Across the Sky, Liu Feng in Beacon Train, Zhang Yong in Hero Tanker, and Guo Tie in Guild Wars.
The same way of expression has also appeared in recent years’ films and TV dramas to resist US aggression and aid Korea. Guan Lei and Zhang Fei in The Sacrifice, the Wu brothers in The Battle at Lake Changjin, and even Li Changshun in The 38th Parallel, although the color of individual heroism has gradually given way to the fraternal revolutionary friendship, the breakthrough point of these works is still the close-up of first-line combat heroes or the group portrait of company of heroes, which reflects the overall appearance of volunteer soldiers.
In this sense, Crossing the Yalu River is a work that breaks through the tradition and returns to it. From the drama version to the film version, Crossing the Yalu River is the first of its kind, introducing the first generation leaders’ judgment and decision on the war situation in new China to a large extent, depicting the desperate struggle on the Korean battlefield and the fierce game in international politics from an unprecedented panoramic perspective in Shangganling, placing the war of founding the country in the historical background and the global situation, fully opening up the horizon and opening up the pattern, so that the audience can have a more comprehensive, deeper and more accurate understanding of this war. The film version looks back at the beginning and end of the war from the perspective of Peng Dehuai, commander and political commissar of the Volunteer Army, and its narrative perspective, pattern and boldness are unprecedented.
 Artistic technique:
From rendering to documentary
How to make fiction on the basis of authenticity is also an unavoidable proposition for war writers. In order to shoot "Shangganling" well, the film crew interviewed more than 100 volunteers veterans who participated in the battle that year and compiled hundreds of thousands of words of materials. Ba Jin, the author of Reunion, the original novel of Heroes and Children, went to North Korea in 1952 to experience the life of volunteers, ate and lived with the soldiers, and even went to the front to interview regardless of his life. From the feedback of many veterans, it can be seen that the great success of Shangganling and Heroes’ Children is largely due to the restoration of the real war environment, not only being as immersive as possible in the shooting scene, but also the casting criteria of Zhang Zhongfa and Wang Cheng have changed from the traditional "hero face" to a more grounded "all beings".
It can’t be ignored that the two films originated from reality and are higher than reality, and their powerful artistic appeal transcends the barrier of the crowd and the limitation of time and space. My Motherland, the episode of Shangganling, and Ode to Heroes, the two red golden songs, are still widely sung today. Wang Cheng’s line "Fire at me for victory" has become a highly concentrated revolutionary spirit. It should be noted that the energy of art lies in inspiring patriotic feelings flowing in the blood of the nation.
After more than 60 years, the theme of resisting U.S. aggression and aiding Korea has been revived, and authenticity is also vitality, but the way of reproduction can no longer be reproduced. From the perspective of the witnesses, many people who shed their blood and sweat on the Korean battlefield are old or dead, and today’s Korean peninsula has long been another scene. From the receiver’s point of view, the mainstream audience was born in peacetime and had no personal experience of the war years. Therefore, today, with the great improvement of scientific and technological means, it is as important as artistic rendering of feelings to arouse the national war memory with documentary details.
For example, in the TV series "Meritorious Works", the vivid details such as the operational meeting, the "three-three system" charge formation, the scattered mortar placement, and the artillery blockade law of the US military made the military fans hooked up. These seemingly unremarkable settings and bridges can naturally lead the audience into the raging flames of war without more rendering, and can also strongly refute the narrow argument of "winning the war only by sea tactics" through the lens language, showing the combat courage and wisdom of our soldiers in the case of obviously unequal equipment strength.
Thinking about war:
From sensibility to rationality
From Shangganling, Heroes and Children to Crossing the Yalu River, The Battle at Lake Changjin, there are great generational differences in narrative angle and artistic techniques in the film and television dramas on the theme of resisting US aggression and aiding Korea. Behind this difference, in fact, two generations are thinking and questioning the war to resist US aggression and aid Korea from different angles. The question raised by early film and television dramas is: Why can you win? Now, before answering this question, we have to ask: Why do you want to fight?
Crossing the Yalu River is substituted into the perspective of the first generation of new China leaders, which overlooks the overall situation from a strategic perspective and explains the urgency of the war for the founding of the country with historical vision and political language. In Li Yannian, Li Yannian made a speech about home and country when he was dealing with the deserter Anton. This speech radiated the overall situation from emotional dimension and described the necessity of defending the country in popular words.
Explaining "why fight" is based on the dual needs of history and reality, and gives an exact footnote to this endless war, completing an important puzzle for the younger generation’s view of history. The explanation of "why fight" is also based on the gradual transition from sensibility to rationality. After a lapse of 70 years, we will face up to the war and face history with a calm strategic mind.
Of course, rational thinking about the war also means that works of art have more yuan and more subtle interpretations of the part of the war on the premise of keeping the main tone generous and high. For example, The Battle at Lake Changjin Zhong Wu Qianli’s line that "some guns must be opened, and some guns can’t be opened", and Li Yannian’s bridge in "Merit" doesn’t kill deserters, all of which jump off from the traditional routines of war film and television dramas and tap the shining human brilliance in the people’s army in detail.
From the TV series "The 38th Parallel" to the movie version "Crossing the Yalu River", many high-quality works have come out one after another, bringing the theme of resisting US aggression and aiding Korea back to the center of the film and television industry. For the audience, the grand narrative constructed by these works is not only an art movement driven by new technology, but also a historical education called for by the new era.
(Source: Wen Wei Po)
Reporting/feedback

The director of the Alashan League Cultural Tourism Bureau was dismissed, and more than half of the stranded tourists in Ejina Banner have returned.

After the outbreak of this round of epidemic, many officials in Alashan area of Inner Mongolia Autonomous Region have been held accountable for "ineffective performance of duties" in epidemic prevention and control, and the secretary of Ejina Banner Committee and the director of Alashan League Cultural Tourism Bureau have been dismissed successively.
According to the official micro-signal of Alashan Daily on October 30th, Comrade Sun Jianjun failed to implement the deployment requirements of epidemic prevention and control in the country and autonomous region in time, failed to perform his duties, and failed to promote the work related to epidemic prevention and control, and was removed from his post as Party Secretary and Director of the Bureau of Culture, Tourism, Radio and Television of Alashan League and Director of the Cultural Relics Bureau of the League.
This round of epidemic since the 18th of this month has interrupted the tourist season in Alashan League, and the return of stranded tourists has become the focus of epidemic prevention work in Alashan League and even in Inner Mongolia. As of the afternoon of the 29th, Ejina Banner, the "hardest hit area" of Alxa League epidemic, has transported 5095 local stranded tourists back in batches.
The epidemic interrupted the tourist season, and the return of tourists became the focus of Alashan League.
League and Banner are unique administrative divisions in Inner Mongolia Autonomous Region, which are equivalent to prefecture level and county level respectively. Ejina Banner is a subordinate banner of Alashan League, and it is also the "hardest hit area" in Alashan area at present.
According to the information of official website, the Health and Health Commission of Inner Mongolia Autonomous Region, from 0: 00 to 24: 00 on October 29th, there were 19 newly confirmed local cases, including 18 cases in Ejina Banner of Alashan League. In addition, as of 24: 00 on October 29, there were 155 confirmed cases in Inner Mongolia Autonomous Region, including 132 cases in Ejina Banner of Alashan League.
October is the best viewing period of Populus euphratica forest, and Ejina Banner also welcomes the tourist season. The sudden epidemic since the 18th of this month has forced the golden period of Ejina Banner tourism to be interrupted, and a large number of tourists from other provinces are stranded in the local area.
On the afternoon of October 26th, Alashan League held a press conference on COVID-19 epidemic prevention and control. As of 15: 00 on the 25th, there were 9,412 tourists stranded in Ejina Banner, including 6,024 group tourists and 3,388 individual tourists. The main tourist sources of stranded tourists are Sichuan, Anhui, Jiangsu, Shanghai, Gansu, Guangdong and other places.
After this outbreak, promoting the return of stranded tourists has become the focus of epidemic prevention work in Alashan and even the whole Inner Mongolia.
On October 25th, Wang Lixia, Deputy Secretary of the Party Committee of Inner Mongolia Autonomous Region and Chairman of the Autonomous Region, presided over a dispatch meeting on the site disposal of COVID-19 epidemic prevention and control in Ejina Banner, Alashan League, demanding that every effort be made to meet the reasonable needs of stranded tourists in Ejina Banner.
On 26th, Wang Lixia once again pointed out that the CPC Central Committee and the State Council attached great importance to the service guarantee for stranded tourists and the organization of return trips. The Party committee and government of the autonomous region have made comprehensive arrangements for this, and the key is to implement it strictly, accurately and efficiently.
According to the Inner Mongolia Daily, at 6: 30 in the morning of October 29th, Wang Lixia led Ouyang Xiaohui and Heng Xiaofan, leaders of the autonomous region, to the Ejinaqi toll station of the G7 Expressway, and together with Li Bin, head of the working group of the the State Council Joint Prevention and Control Mechanism and deputy director of the National Health and Wellness Commission, inspected and guided the return work of stranded tourists on the spot.
On October 29, Shi Taifeng, secretary of the Party Committee of Inner Mongolia Autonomous Region and director of the Standing Committee of the National People’s Congress, presided over the dispatching meeting to deal with the epidemic situation in Ejina Banner, stressing that "the stranded tourists should be treated as relatives, and the transshipment work should be done in a closed-loop, safe and orderly manner. One person and one policy should make specific arrangements, strictly implement the practical work plan for return transshipment, and strengthen communication and docking with tourists’ return destinations, so that tourists and all parties can feel at ease."
Meet four health conditionsTransshipment, more than half of the stranded tourists in Ejina Banner have returned in batches.
In the past ten days, Ejina Banner has issued a number of open letters to stranded tourists, informing the local epidemic prevention and control situation and return arrangements, and appeasing the emotions of stranded tourists.
On October 26th, Alashan League Tourism Bureau began to publish the Return Information Form for stranded tourists, collecting information such as tourists’ identity information, return destinations and detailed accommodation addresses, so as to promote the return.
According to CCTV news, at 9: 52 pm on October 27th, the first batch of 586 tourists stranded in Ejina Banner were transported out of Ejina for Zhengzhou by special tourist train.
On the afternoon of 28th, at the second press conference of COVID-19 epidemic prevention and control work held in Inner Mongolia Autonomous Region, Zhou Qiang, deputy director of the Transportation Department of Inner Mongolia Autonomous Region, introduced that up to then, 4 trains of stranded passengers and 2,428 stranded tourists had been transported through the railway tourist special train, striving to transport most tourists out of Ejina Banner in 3-5 days. Passengers whose health conditions do not meet the transfer requirements will stay for a while.
According to Inner Mongolia Daily, on the morning of October 29th, the first batch of chartered tourists from 113 tour groups set foot on their way back, and they will go to the centralized isolation point in Baotou for unified isolation.
On the afternoon of 29th, the press conference of COVID-19 epidemic prevention and control held in Inner Mongolia Autonomous Region informed that at present, the tourists who are transported out of Ejina Banner need to meet four health conditions.
First of all, they must be non-positive, non-close-contact and sub-close-contact, and have carried out centralized health monitoring and 4 nucleic acid tests in Ejina Banner for more than 7 days. Secondly, COVID-19’s clinical symptoms such as fever and dry cough did not appear in the past 14 days. Thirdly, adhere to the principle of voluntariness, abide by the relevant provisions of the state on centralized isolation, medical prevention and control observation, closed-loop management requirements in the process of isolation and transshipment, and relevant provisions on centralized observation and isolation management of destinations. Finally, the nucleic acid test results within 48 hours were all negative.
The press conference also informed that as of the afternoon of the 29th, Ejina Banner had transferred 5095 stranded tourists. So far, more than half of the nearly 10,000 tourists stranded in Ejina have returned.
Due to the ineffective prevention and control of the epidemic, many officials in Alashan have been dismissed.
After the outbreak of this round of epidemic, many officials in Alashan region have been held accountable because of the "ineffective performance of their duties" in epidemic prevention and control, and the secretary of the Ejina Banner Committee and the director of the Alashan League Cultural Tourism Bureau have been dismissed successively.
On October 23, the Committee of Ejina Banner of the Communist Party of China issued a circular, stating that since the local COVID-19 epidemic broke out in Ejina Banner on October 17, some departments and units, party member cadres and staff still turned a deaf ear to the epidemic prevention and control work, failed to perform their duties and failed to implement prevention and control measures, which affected the overall situation of prevention and control work.
The circular specifically pointed out three problems: the problem that the relevant responsible persons of Ejina Banner Health and Wellness Committee did not fulfill the responsibility of epidemic prevention and control; Fan Jianyong, Jiao Jia and Xie Zonglong of Ejina Banner Public Security Bureau failed in their duties, and Ejina Banner People’s Hospital failed to implement the responsibility of epidemic prevention and control; The Civil Affairs Bureau of Ejina Banner failed to implement the epidemic prevention and control measures.
In the notification, Qujiang Hui, the former party secretary and director of Ejina Banner Health and Health Committee, and Liu Qin, the former party member and deputy director, were dismissed, and Tunamula, the party member and deputy director of Ejina Banner Health and Health Committee, was dismissed by informed criticism.
On October 26th, Inner Mongolia Daily reported that in order to compact the territorial responsibility of epidemic prevention and control and intensify the front-line prevention and control work, the Party Committee of Inner Mongolia Autonomous Region decided that Dai Qin, secretary of Alxa League Committee, would also serve as secretary of Ejina Banner Committee. Comrade Chen Zhanyun was removed from the post of secretary of Ejina Banner Committee because of his conscientious performance and ineffective implementation in epidemic prevention and control work.
Alashan Daily’s official micro-signal "Alashan Today" was reported on October 30th. According to the research decision of the Alashan League Committee, Comrade Sun Jianjun failed to implement the deployment requirements for epidemic prevention and control in the state and autonomous regions in time, failed to perform his duties effectively, and the work related to epidemic prevention and control was not in place. He was removed from his post as Party Secretary, Director and Director of the Bureau of Culture, Tourism, Radio and Television of Alashan League.
According to public information, Sun Jianjun has been working in Alxa League, Inner Mongolia since he joined the work in 1991. Since January 2019, Sun Jianjun has served as Party Secretary and Director of the Bureau of Culture, Tourism, Radio and Television of Alxa League, Inner Mongolia Autonomous Region.
Beijing News reporter Hu Xianhe
Editor Zhan Shengjie proofreads Wu Xingfa
Reporting/feedback

"Pure Blood Hongmeng" is on its way! Hongmeng Pioneer App Zone is officially launched on Huawei Store

Fast technology news on December 30, this September, Huawei announced that the new HarmonyOS NEXT is ready to go, and Hongmeng native applications are fully launched.

A few days ago,"Hongmeng Pioneer Application Zone" is officially launched in the Huawei app store. These applications are placed in the Hongmeng Pioneer Application Zone to represent that Hongmeng native application development is under development or has been completed.

It is reported that there are currently around 150 applications that have launched or completed the development of Hongmeng native applications.

These include Autonavi Maps, QQ Music, iQIYI, Himalayas, QuickPass, Douyin, 360 Mobile Browser, Alipay, Meituan, Jinri Toutiao, Xiaohongshu, Weibo, Kugou Music, Bilibili, Dragonfly FM, China Construction Bank, Flush, Romance of the Three Kingdoms: Strategy Edition, and more.

It should be reminded that the current applications in the Hongmeng Pioneer Application Zone are all promoted in advance and play a showcase role. If you want to experience the native application of Hongmeng, you still have to wait until 2024.

It is understood that,The developer preview of HarmonyOS NEXT, known as "Pure Blood", will be available to developers in Quarter 1, 2024.

With the launch of HarmonyOS NEXT next year, users will also truly experience a smoother, smarter, and safer "Pure Blood Hongmeng" application, which is worth looking forward to.

"Pure Blood Hongmeng" is on its way! Hongmeng Pioneer App Zone is officially launched on Huawei Store

(Report)

The latest inventory | How many stem cell drugs have been listed in the world?

At the 2020 China Biotechnology Innovation Conference, experts pointed out that stem cells, as strategic resources, are strategic, public-spirited and supportive, in which the strategy is for national security and future competition, the public-spirited is for population health and social development, and the supportive is for scientific, technological, industrial and economic development. It is an important measure to ensure people’s health to collect, store, develop, utilize and share strategic stem cell resources and promote clinical transformation.

Stem cell resources are important strategic resources of the country. How to safely and effectively preserve China’s special, unique and rare human biological resources is of great significance for promoting China’s population health, maintaining the safety of life resources and treating major diseases.

One of the important values of stem cells is their application in disease treatment.

Professor Thomas C. Sudhof, winner of the Nobel Prize in Physiology or Medicine, once said, "We can control the disease and stem cells are a very good treatment. " Stem cell research will bring new hope to the problems that traditional medical treatment can’t solve.. "

Internationally, the United States, South Korea, Japan, the European Union and so on have approved stem cell therapy products listed, and stem cell therapy for diseases is becoming a more extensive reality.

By April, 2021, more than 1,200 clinical trials of mesenchymal stem cells were retrieved from ClinicalTrials.gov website.

In recent years, this number has continued to increase, indicating that the importance of mesenchymal stem cells is increasing.

At present, the research and development of stem cell drugs in China is becoming more and more standardized, attracting a lot of capital and talents. At the moment when the results are about to blow out, we look forward to this market by combing the foreign mesenchymal stem cell drugs.

According to incomplete statistics, there are 18 mesenchymal stem cell products that have been approved for marketing in the world, and 10 products meet the definition of drugs.

18 products related to mesenchymal stem cells (including orphan drugs, conditionally approved drugs, medical products, etc.); The date and country of approval are the first approval; Multistem is not counted, because its official website claims that the drug is not mesenchymal stem cells.

01

More flowers, Mesoblast

Prochymal: graft versus host disease

Country: USA, Canada, New Zealand

Date: May 2010

Cell type: bone marrow mesenchymal stem cells

Indications: graft versus host disease, Crohn’s disease

Price: $200,000/course of treatment

Initially, the FDA of the United States approved it as an orphan drug for the treatment of type I.

In 2012, Prochymal was sold to Mesoblast in Australia, and was approved for marketing in Canada and New Zealand to treat acute graft-versus-host disease.

In 2015, Mesoblast cooperated with JCR and renamed it Temcell to be listed in Japan.

Temcell: graft versus host disease

Country: Japan

Date: February 2016

Cell type: bone marrow mesenchymal stem cells

Indications: graft versus host disease

Price: about $120,000/course of treatment.

As mentioned above, Temcell was formerly Prochymal of Osiris Company and Ryoncil of Mesoblast Company.

In 2019, TEMCELL was approved to treat epidermolysis bullosa and neonatal hypoxic-ischemic encephalopathy.

In JCR’s financial report, Temcell’s sales revenue was about 18 million US dollars in 2018 and 28 million US dollars in 2019.

MPC: bone repair

Country: Australia

Date: July 2010

Cell type: autologous mesenchymal precursor cells

Indications: bone repair

Price: unknown

In 2010, the MPC(Mesenchymal Precursor Cell) independently developed by Mesoblast was approved for listing in Australia for the repair and regeneration of damaged tissues.

Mesoblast’s current market value is about $1 billion, and its business income of $6.6 million in fiscal year 2020 comes entirely from Temcell, mainly from milestone payments ($25 million) from China, Japanese and other partners.

02

Try first, Korea FDA

CellGram: acute myocardial infarction

Country: Korea

Date: July 2011

Cell type: autologous bone marrow mesenchymal stem cells

Indications: Acute myocardial infarction

Price: $15,000

CellGram was used by coronary angioplasty within 72 hours after chest colic to improve the left ventricular blood cell count in patients with myocardial infarction. After 6 months of treatment, the measurement showed that each treatment improved the left ventricular ejection fraction by 6%.

By 2017, this therapy has created sales of about $7.8 million. However, CellGram failed to meet the requirements of treating 600 patients within a six-year period, and Pharmicell applied to reduce the minimum number of patients to 60, which was rejected.

Some industry observers speculate that South Korea will cancel its approval, but this is the first stem cell drug in South Korea. "The cancellation of approval will have a harmful impact on the national reputation, and we must make a cautious decision."

Cartistem: degenerative arthritis

Country: Korea

Date: January 2012

Cell type: umbilical cord blood mesenchymal stem cells

Indications: Degenerative arthritis and knee cartilage injury.

Price: 19,000-21,000 USD

In the eight years since Cartistem was put on the market, the cumulative sales have exceeded 18,000 doses.

From 2017 to 2020, Cartistem has sold more than 10 billion won (about 9 million US dollars) for four consecutive years, which is obviously better than the other three stem cell drugs approved by South Korea (the annual sales are less than 5 billion won).

Medipost quarterly sales trend (millions of won, source: Medipost)

Researchers such as Kim SJ conducted a retrospective analysis of 128 product users, and found that after two years of follow-up, the pain and function of these patients were improved, which was statistically significant.

At present, the product has completed phase I and IIa clinical trials in the United States, and phase II clinical trials are being carried out in Japan.

Cupistem: Crohn’s disease

Country: Korea

Date: January 2012

Cell type: autologous adipose-derived mesenchymal stem cells

Indications: complex Crohn’s disease complicated with anal fistula

Price: 3000-5000 USD

Cupistem is used to reduce inflammation of Crohn’s fistula.

In a phase II clinical trial, 43 patients were evaluated for fistula healing after 12 months, and 80.8% of them showed complete fistula healing. After 24 months, 75% of these patients were completely closed, showing sustainable safety and therapeutic effect.

NeuroNATA-R: amyotrophic lateral sclerosis

Country: Korea

Date: July 2014

Cell type: autologous bone marrow mesenchymal stem cells

Indications: amyotrophic lateral sclerosis, motor neuron disease

Price: USD 18,000-72,000 (according to weight)

NeuroNATA-R is a stem cell therapy provided by Corestem Company of South Korea. Mesenchymal stem cells need to be collected from the patient’s own bone marrow four weeks before each injection of stem cells.

In the phase I clinical trial to evaluate the safety, 7 patients received intrathecal injection twice and were followed up for 12 months. The research shows that it has good security.

03

Not to be outdone, Japan and India

Stempeucel: Severe limb ischemia

Country: India, ratified by EU.

Date: June 2015

Cell type: bone marrow mesenchymal stem cells

Indications: thromboangiitis obliterans

Price: $2,200 (2017)

In 2015, Stempeucel was approved by the European Medicines Agency (EMA) as an orphan drug to treat thromboangiitis obliterans.

In 2017, the General Administration of Drug Administration of India (DCGI) granted Stempeucel a limited marketing license to treat patients with severe limb ischemia caused by Buerger’s disease. In 2020, DCGI completed the formal approval.

The research and development of Stempeucel lasted for 12 years and finally became the first allogeneic cell therapy product approved for commercial use in India.

Alofisel: Crohn’s disease

Country: Japan, EU ratification

Date: March 2018

Cell type: adipose-derived mesenchymal stem cells

Indications: complex perianal fistula in patients with Crohn’s disease

Price: about $70,000/course.

In 2018, Takeda, a Japanese pharmaceutical giant, and TiGenix, a Belgian stem cell company, jointly announced that EMA had approved Alofisel (CX 601) for the treatment of Crohn’s disease complicated perianal fistula whose fistula failed to respond to at least one traditional or biological therapy.

However, Alofisel encountered some difficulties in obtaining the so-called third-level approval, which is a term used to refer to national payer insurance. For example, NICE in the UK refused to recommend Alofisel on the grounds that it did not benefit much from clinical trials.

In 2019, Takeda decided to adopt value-based pricing in Europe. In other words, if patients do not improve, they will bear part or all of the expenses.

In the fiscal year ending March 31st, 2020, Takeda earned about $350,000 from Alofisel.

Stemirac: spinal cord injury

Country: Japan

Date: 2018-12

Cell type: autologous bone marrow mesenchymal stem cells

Indications: spinal cord injury

Price: $135,000

The listing of Stemirac is somewhat controversial. It only did phase II clinical trials, and it was conditionally approved and included in the national health insurance.

This move was criticized by Nature, who thought that there was no control group in its clinical trial, and there were only 13 subjects in the treatment group. The Japanese side responded that setting up a control group may cause moral problems.

According to an article in 2019, Stemirac will bring about $3.4 million in revenue to Nipro every year in the next nine years.

04

The pace of clinical treatment of stem cells in China is accelerating!

According to the data of National Medical Products Administration Drug Evaluation Center, up to now, eight new drugs of mesenchymal stem cells have been applied for clinical approval in China, and the indications are acute graft-versus-host disease, rheumatoid arthritis, ischemic stroke, knee osteoarthritis and diabetic foot.

Source: CDE

At present, the supervision of stem cell therapy in China adopts a "dual-track management model". National Health Commission mainly manages clinical research conducted by medical institutions, and the Food and Drug Administration is mainly responsible for stem cell therapy products that are applied for marketing after clinical trials are completed according to drugs.

In recent two years, the clinical application of stem cells in China has been standardized under the "dual-track management model".Up to now, 74 stem cell clinical research projects have passed the record of National Health Commission. Eight applications for new stem cell drugs have been approved by the Food and Drug Administration..

Compared with the past few years, the clinical transformation of stem cells in China is developing with a brand-new trend, and the blank of domestic stem cell listed drugs is expected to be filled faster.

At present, in the field of stem cell transformation, the research in China mainly focuses on stem cell bank, stem cell drug application, preclinical research and clinical trials. The gradual improvement of regulatory policies has pushed the clinical transformation of stem cells in China to a new development situation.

First, China has entered a new stage of development of new stem cell drugs.The promulgation of "Technical Guiding Principles for Research and Evaluation of Cell Therapy Products (Trial)" clarified the standard of stem cell therapy as a drug declaration, and at the same time, the new drug approval system of "60-day clinical trial implied permission" provided new development opportunities for the research and development and declaration of new stem cell drugs in China.

Second, the national "stem cell and transformation research" focuses on promoting the development of stem cell therapy drugs.For example, one of the assessment objectives pointed out in the key special declaration guidelines is to develop cell therapy drugs for a major or rare disease, apply for registration of new stem cell drugs and apply for clinical approval of stem cells.

Third, the policy encourages the strength of comprehensive enterprises to promote the clinical transformation of stem cells.In the past two years, some domestic policies explicitly support qualified 3A hospitals to cooperate with qualified social forces to declare and carry out stem cell clinical research, that is, to support and encourage qualified social forces or preparation institutions (enterprises) to join the stem cell industry and jointly promote the clinical application and development of stem cells. The promotion of enterprise innovation forces has accelerated the development and application of stem cells in China.

At present, there are still many diseases that need innovative therapies to solve the unmet clinical needs, and the expansion of indications for new stem cell drugs is precisely to meet these needs.

05

Perinatal tissue stem cells have gradually become mainstream.

Perinatal tissue is one of the largest sources of mesenchymal stem cells, and it has become a popular source of stem cells for new drug research and development. Mesenchymal stem cells derived from perinatal tissues have the advantages of multi-directional differentiation potential, immunological characteristics, convenient and harmless material collection, wide sources and easy industrial preparation, which has become a new trend of medical development. At present, more than 60% of the new stem cell drugs that have obtained clinical approval in China use mesenchymal stem cells derived from perinatal tissues.

In the treatment of severe patients in COVID-19, mesenchymal stem cells derived from perinatal tissues have also shown a good effect. Domestic research reports show that the critically ill patients in COVID-19 were transferred out of ICU after 9 days of reinfusion of mesenchymal stem cells from perinatal tissues, and most vital signs and clinical trial indicators returned to normal, and both throat swab tests were negative for Covid-19.

In recent years, immune cells derived from perinatal tissues have gradually become a research hotspot. Previously, the FDA approved the clinical trial of immune cells derived from human placental stem cells to treat COVID-19, which was developed by Celularity, a biotechnology company in New Jersey, USA.

In the early trial, as many as 86 patients with symptomatic COVID-19 will be treated to evaluate the safety of the treatment and the ability to prevent patients from getting worse.

Internationally, placental cell therapy products are also accelerating the pace of entering the market. For example, placenta-derived adherent cell PLX-PAD, which has attracted much attention in recent years, is being designed to treat patients who do not need tissue or genetic matching.

This therapy has passed the accelerated approval routes in the United States and the European Union, including the quick approval qualification of the US FDA and the Adaptive Pathways program of the EU EMA, for the treatment of limb ischemia. In addition, PLX-PAD has also been used in clinical research of other indications, including intermittent claudication, orthopedic indications, pulmonary hypertension and women’s health, and some of them have entered clinical phase III.

At present, China’s stem cell industry has formed a huge market scale. With the clinical transformation and market development, the era of stem cells benefiting patients in China will also come.

Wrists rub against each other after wearing perfume? It turns out that the way to use perfume for so many years is wrong.

  If you are annoyed that your expensive perfume doesn’t smell so good in a bottle after being sprayed on your body, or the fragrance disappears within an hour after you leave home, then there may be something wrong with the way you use perfume.

  Francis Courgian, a famous French perfumer who has made popular perfumes of Dior, Burberry, Lancome and other famous brands, recently revealed five misunderstandings of women’s use of perfume in an interview with Vogue magazine.

  Kurgian pointed out that to make perfume release the best taste, you must consider the environment in which you use perfume and the clothes attached to it. Not only that, if you are not used to wearing perfume every day, you’d better buy a small bottle, because the shelf life of perfume is usually only three months. As for how to use perfume correctly, let’s listen to what this perfumer has to say.

  If you’re in the habit of spritzing your wrists and then rubbing them together, you’re actually ruining the scent.

  If you are used to spraying perfume on your wrists and rubbing them against each other, you are actually destroying the fragrance.

  That’s because the friction "heats up the skin, which produces natural enzymes that change the course of the scent", with the top and middle notes being worst affected as well as the dry-down – the long period during which the fragrance unfolds.

  This is because friction "will make the skin warm, thus producing natural enzymes that will change the fragrance stage", and the former and middle tones are most affected, and the latter tone-the long process of fragrance release-will also be affected.

  If you’re wearing a floral perfume, for instance, this heat makes it lose its crispness.

  If you use floral perfume, the heat will make it lose its fresh taste.

  To keep your fragrance smelling like it should, as well as making it last longer, spritz it once and then simply let it sink in.

  In order to make your perfume give off its beautiful fragrance and make it last longer, you just need to spray it and let the perfume penetrate into your skin by itself.

  Don’t DON’T KEEP IT IN THE BATHROOM.

  Perfume is extremely sensitive to environmental changes and will be impacted by changes such as going from cold to hot.

  Perfume is extremely sensitive to external changes and will be affected, such as the temperature changing from cold to hot.

  This process sets off "unexpected chemical reactions within the natural ingredients" of the perfume such as patchouli, meaning your perfume will age faster and start to smell "off".

  Temperature changes will trigger "unexpected chemical reactions between natural ingredients (such as patchouli)" of perfume, which means that your perfume will "age faster" and start to "taste bad".

  The best way to store your fragrance is in its original box at room temperature.

  The best way to store perfume is to put it in an original box and put it in a room temperature environment.

  Dooopt for small sizes chooses perfume in small bottles.

  If you are lucky enough to have lots of bottles or you don’t use your favourite perfume every day, oxygen will start to break down the molecules as it languishes on your dressing table.

  If you are lucky enough to have many bottles of perfume, or if you don’t use your favorite perfume every day, then oxygen will start to break the molecular structure of perfume and make it gradually evaporate and deteriorate on your dressing table.

  A typical bottle has a shelf life of three months so if you’re not using a perfume daily, you’re best off buying a smaller variety.

  The shelf life of a typical perfume is three months, so if you don’t use perfume every day, you’d better buy a small bottle.

  Or you could decant your bigger bottles into smaller containers and keep them in the fridge to maintain perfection.

  Or you can pack your big bottle of perfume into several small bottles and put them in the refrigerator to keep the perfect fragrance.

  DO EMBRACE SYNTHETICS chooses perfume with artificial ingredients.

  The idea of a perfume that boasts of being all-natural might sound appealing, but the fragrance may well let you down.

  Perfume under the banner of pure nature may sound attractive, but the fragrance of this perfume will disappoint you.

  Musk, which was formerly derived from animals, is probably the most popular fragrance ingredient but must be created synthetically.

  麝香很可能是最受欢迎的香水原料,过去取自于动物,现在只能人工合成。

  Other popular smells, such as peony, freesia, and lily of the valley, can’t be extracted naturally as they don’t release enough aroma so they must be re-created.

  其他受欢迎的香味,比如芍药、小苍兰、铃兰,不能通过自然手段提取,因为这些花释放的香味不足,所以必须人工再造。

  According to Kurkdjian the ideal formula is a blend of essential oils, absolutes, and man-made ingredients.

  Kurgian pointed out that the most ideal perfume formula is a mixture of essential oil, purified oil and artificial raw materials.

  DO SPRITZ IT ON YOUR HAIR spray perfume on your hair.

  Perfume actually doesn’t last too long on dry skin, according to Kurkdjian and as you sweat the natural oils destroy the fragrance.

  Kurgian pointed out that perfume can’t stay on dry skin for too long. When you sweat, natural oils will destroy the fragrance.

  You can combat this by using the matching body lotion or making sure your moisturiser isn’t scented so it doesn’t interfere.

  You can prevent the fragrances from interfering with each other by using a body lotion that matches the scent of perfume or a body lotion without perfume.

  If you want to ensure longevity, mist your scent lightly on your hair or on a scarf.

  If you want perfume to last a long time, you can spray it gently on your hair or scarf.

  ’Because they move with the air, it helps with the diffusion of the scent,’ he explained.

  He explained: "Because the hair and scarf are fluttering in the wind, it helps to diffuse the fragrance."

  If you are putting perfume on your skin, target spots that are exposed to the air.

  If you spray perfume on your skin, you’d better spray it on the body parts exposed to the air.

  It’s no good putting it on pulse points such as the wrists and inner elbows if they’re covered up.

  If the wrist or elbow is covered with clothes, it is useless to spray perfume on the pulse beating place such as the inside of the wrist or elbow.

  (English source: Daily Mail Translation & Editor: Dany)

What did you do? The 63-year-old investigated official has studied in Europe and America and vowed to be the best translator in China.

  CCTV News:At 8 o’clock on the morning of December 16th, the website of the Supervision Department of the Central Commission for Discipline Inspection released a message saying that Cao Baijun, the former director of the Fourth Bureau of the International Liaison Department of the CPC Central Committee, was suspected of serious violation of discipline and was currently under organizational review.

      Cao Baijuan was born in Nanjing, Jiangsu Province in April 1954. Graduated from Shanghai International Studies University (now Shanghai International Studies University) in 1975, majoring in German, and worked in the International Liaison Department for a long time.

  In 2009, Shanghai International Studies University celebrated its 60th anniversary. As an alumnus, Cao Baijuan returned to Shanghai to have an exchange meeting with German teachers and students. Cao Baijuan recalled his college life. He said: "There should be three problems to be solved in college. First, study hard. Second, deal with interpersonal relationships. In addition, we should establish correct political beliefs, outlook on life and values." Referring to his life experience, he said that when he graduated from university, his goal was to become the best translator in China. To this end, he did a lot of exercises and read a lot of books. "The goal is very single, so it is easy to succeed." He described himself like this.

  According to public information, Cao Baijun worked in the International Liaison Department of the CPC Central Committee from 1975 to 1982, and was responsible for the business of this department and German-speaking countries in Europe. In 1982, he won a scholarship from the Adenauer Foundation in Germany, and studied in the Institute of Political Science of the University of Heidelberg, Germany. The instructor was Klaus von Baimer; After returning to China in 1985, he served as the director of the German Department of the International Liaison Department of the CPC Central Committee; During this period, I participated in the reception of famous German politicians visiting China, such as johannes rau, La Fontaine, Scharping, Birna, Barr and others, and served as the chief translator. In 1992, he went to UCLA for short-term study.

  From November 1992 to August 1993, he served as the director of the Planning Commission of Shanghai Pudong New Area Government. From August 1993 to May 1999, he served as deputy general manager and general manager of China Hualian International Trade Company; In May 1999, he served as the director of China Economic Liaison Center and concurrently served as the general manager of China Hualian International Trade Company; In 2007, he began to serve as the director of the Fourth Bureau of the International Liaison Department (Africa Bureau) until he left office.

  After leaving office, Cao Baijuan became a company consultant. In August, 2017, a company published a news in official website that a Spanish representative came to visit and investigate, accompanied by Cao Baijuan, a senior consultant of the company. This was the news of Cao Baijuan’s last public appearance.

      It is worth mentioning that from July 2 to August 31, 2016, the Central Fourth Inspection Team conducted a special inspection of the International Liaison Department. During the inspection, the inspection team found some problems reflected by the cadres and the masses, mainly: the "four consciousnesses" are not strong enough, there is a gap in implementing the central decision-making and deployment, and it is not enough to respond to the central concerns and take the initiative; The party building work is weakened, the ideological and political work is weak, the rectification of the party’s mass line education practice activities is not in place, and the implementation of attendance system is not strict; Strictly administering the party in an all-round way is not strong enough, supervision and discipline are loose and soft, and investigation and punishment of some obvious violations of discipline are not in place; There is not enough transparency in the selection and appointment of cadres, and there is a phenomenon of "discussing personal relationship", and the training and exchange of cadres are not enough; Spiritual behaviors that violate the eight central regulations still occur, such as office buildings, overseas delegations, official receptions, etc., and individual subordinate units illegally distribute benefits and subsidies; The management of state-owned assets and funds is lax, the management of real estate rental is chaotic, and there is a risk of clean government. At the same time, the inspection team also received reports of problems involving some leading cadres, which have been transferred to the Central Commission for Discipline Inspection, the Central Organization Department and other relevant parties for handling according to relevant regulations.

  It is unknown whether Cao Baijun is included in the problems involving some leading cadres mentioned in the inspection report.

Telemarketing is "tricky": the intelligent outbound call system claims to be able to make thousands of calls a day and prevent the number from being blocked.

"Congratulations on lending 500,000 yuan, and customers can’t keep up." Xiao Bo (a pseudonym), who claimed to be an intelligent outbound service provider for telemarketing, updated his circle of friends with a screenshot of a customer’s successful lending. In Xiao Bo’s circle of friends, there are many similar "success stories". Xiao Bo claims that his telemarketing robot can make more than 1,000 calls in a single day, and the connection rate remains at 30% or even higher, and can avoid supervision and prevent titles.

Are you still bothered by harassing calls about selling loans and POS machines?

From late October to November, 2021, The Paper reporter found that the chaos in the telemarketing industry still exists. The technical model of "outbound call system+telemarketing robot" claims to be able to accurately dial the special line and mark the customer information, and evade the supervision of communication operators through technology; Behind it are hidden behaviors such as illegal trading of personal information and illegal sales of POS machines.

The so-called "anti-sealing" call-out system for electric sales

Telemarketing is a sales method relying on telephone, which contacts customers through special telephone marketing numbers to complete product promotion, quotation, transaction, etc., but this method is often easy to cause harassment to users. In the media reports, there are many cases in which telephone users are often disturbed by receiving telemarketing calls. In recent years, the Ministry of Industry and Information Technology and the three major operators have taken measures such as "high-frequency call blocking". However, with the application of intelligent voice and other technologies, the intelligent outbound calling software system, which claims to be able to make a large number of calls a day and "prevent blocking" (prevent blocking), still makes users hard to prevent.

A recent survey in The Paper found that some telemarketing software used "anti-sealing" as a gimmick to attract customers, and all kinds of "anti-sealing robots" gradually became popular in the market.

In Baidu Post Bar and Tencent QQ, the keyword "electricity sales" is searched, which shows a lot of information about "electricity sales software". The reporter randomly joined a QQ group named "Outbound Call System for Telemarketing", with more than 1,000 members and an account named "Chamber of Commerce for Telemarketing Systems" posting various promotional information related to Telemarketing from time to time in the group. "electricity sales card", "electricity sales App" and "electricity sales anti-sealing system" ……

The sales information in the group claims that the services provided can cover the whole industry such as finance, collection, loan, insurance, health care, drinks, auto insurance and games.

The reporter randomly added the WeChat of Xiao Bo, a telemarketing intelligent outbound service provider, from the QQ group. He asked the reporter what business he wanted to do, saying, "Let’s see if we can do it. Now the telecom fraud investigation is strict." After confirming that it was a "campus loan" business, he enthusiastically introduced his products and sent a price list, including a set of real-life speech made by 300 yuan, and a robot package divided into basic version, premium version and agent version according to different price points and specifications. During the conversation, Xiao Bo said that most of his clients are selling financial stocks, loan insurance and POS machine sales, all of which can be "sealed".

In his description, the "anti-sealing robot" does not need any calling cards and auxiliary equipment, and the outbound call system purchased together will use the group call line to dial the number. Regarding the reporter’s question that "making too many calls will not be seized", Xiao Bo replied that a certain line will inevitably have complaints every month, but as long as it reaches the upper limit of the complaint rate, it will stop the line and replace it with another one, so as to prevent the system purchased by customers from being blocked. He also mentioned a new business to be launched soon-an anti-sealing App for electricity sales. In the promotional picture of the App, the words "the three major operators’ Puka seconds turn white list and the high frequency does not seal the number" are placed in the most conspicuous position.

In order to further dispel the reporter’s doubts, Xiao Bo added, "We are first-class landing line operators and all cooperate with operators."

He Zheng (a pseudonym), another service provider who claimed to be engaged in the research and development of the telemarketing system for 6 years, explained to reporters that the telemarketing robot business on the market now basically adopts the "line+system+service" model. Buyers only need to use the account they provide, and they can enjoy a full set of services, "including technical support, carrying lines and after-sales."

In the 1 minute and 22 second recording of "Loan Talk" he sent, a customer on the other end of the phone in love women, who claimed to be a "customer service representative of a bank", indicated that he could handle a loan of 3-5 million yuan and "pay it back as you borrow it". In this conversation, the customer service representative repeatedly said that he was a staff member of "a bank credit center" and asked about the occupation of the telemarketing customer, the loan and insurance business he handled, and "whether there is a house or a car under his name".

He Zheng said that the so-called "bank customer service representative" bought their telemarketing system, recorded it in advance, and combined it into a complete sales talk according to the matching of the system. The customer thought it was a real person and was constantly "promoted", and might place a loan order. But in fact, they don’t know whether this "bank customer service representative" is a bank staff member or a telecom fraudster.

The "material dealer" in the dark of the electricity sales industry

In the telemarketing industry, an important part is to obtain customer information resources. The illegal trading of personal information also breeds in the dark.

After the reporter joined a QQ group, a "supplier" who claimed to specialize in health care lines (the person who provided information such as the user’s phone number) sent a message in the QQ group saying that he obtained the information of consumers who bought a health care book in TV shopping programs and then sold it to the telemarketing team or system service provider who promoted health care products. In the screenshots he sent, these consumers are distributed in different provinces such as Shandong, Henan and Zhejiang.

Compared with system service providers, "material suppliers" are more cautious. They usually don’t directly give contact information such as WeChat or telephone, and they will communicate further after confirming that the other party is an "intended customer".

In a chat with a "supplier" named AZY, the reporter learned that most customer resources are divided into "AI materials" and "hand-stripping materials". "AI material" is provided for electric sales robots, while "hand stripping material" is provided for employees who make phone calls manually. According to the feedback rate of dialing, the prices of the two "materials" are also different. Generally speaking, the feedback rate of "hand stripping" is higher than that of "AI material" and therefore the price is higher.

According to the price standard sent by AZY, 600 yuan RMB can buy 10,000 pieces of AI materials or 1,000 pieces of user information. These materials are usually replaced by different pinyin abbreviations, such as the special line for investors, which they call "GM powder", and the online loan, which is called "Wd".

There are many "anti-pit and anti-second-hand material merchants" posts in Baidu Post Bar. Some familiar "material merchants" will add WeChat to each other and set up their own WeChat groups, in order to expand or stabilize their sources of "materials".

In the investigation, the reporter found that in order to be more concealed, the transactions between suppliers and buyers often shift from WeChat to other more private online trading platforms. The line operator "AXY" chose an overseas chat software called "Telegram" to complete the transaction with the customer. After failing to reach a transaction with the reporter, "AXY" immediately closed his QQ space.

How to deal with the chaos of electricity sales

In recent years, the phenomenon of electricity sales has been repeatedly concerned and rectified.

In 2019, the central bank issued document [2019] No.85 "Notice of the People’s Bank of China on Further Strengthening the Management of Payment and Settlement to Prevent New Crimes in Telecommunication Networks", pointing to the chaos of telephone sales of POS machines. At the same time, a number of payment institutions issued a notice on strictly prohibiting online sales and electronic sales of POS machines. However, the circle of friends of service provider He Zheng is still full of sales records of a large number of "POS machine" sales customers. When the reporter asked about the POS machine sales business, he did not show him any business license in the financial industry.

According to media reports, the sales scam of POS machines has already become a high-incidence area for complaints. Telephone sales mostly attract customers to use their products with "low rate and free delivery", but after the user places an order, there will be various charges including activation fee, deposit, service fee and so on. The reporter’s search on the Internet also found that many deceived users reported that they could not get the account after receiving the POS machine, and even were blacked out directly by the salesman.

In May this year, the Consumer Protection Bureau of China Banking and Insurance Regulatory Commission issued Circular No.8 of 2021, "Circular on Cases of Ping An Bank’s Telemarketing of Physical Products Infringed on Consumers’ Legal Rights and Interests" (hereinafter referred to as "Circular"), which informed the case that Ping An Bank cooperated with a third party to sell physical products by telephone and infringed on consumers’ legal rights and interests.

According to the "Circular", in the spot check of 60 physical products sold by Ping An Bank Credit Card Center, 53 businesses were illegal, accounting for 88.33%.

Fu Liang, a telecom analyst, told The Paper that at present, the management of the domestic electricity sales industry has not yet formed a complete norm. "Many links have not been (completely) opened." If the management standards between relevant departments cannot be unified, there will be barriers to the management and rectification of the chaos of electricity sales. Even if users find their personal information leaked or traded, it is often difficult to find a "channel" for complaints and handling.

Fu Liang also pointed out that operators are the primary link to ensure that users’ information is not leaked. They often have their own strict operating norms, but they cannot completely avoid the possibility of leaks in huge business branches. Moreover, in this highly developed network era, many personal information is in a "semi-public" state.

He believes that in the use of various scenes in daily life, such as telephone numbers and names, information is constantly used and exchanged, and it is difficult to completely quantify whether it involves "privacy disclosure". "If there is an improper transaction of user information, no matter where it happens, it is an illegal act."

Struggle for the Cause of Poverty Alleviation to the Last Moment —— In Memory of Huang Xing, Deputy Director of Shiyan Poverty Alleviation Office, Hubei Province

  Knowing the news of Huang Xing’s death, Yang Chengrong, a poor household who was nearly 80 years old, burst into tears: "He is a relative of our poor household!"

  Huang Xing, deputy director of Shiyan Poverty Alleviation Office, Hubei Province. On February 28th this year, 46-year-old Huang Xing suffered a sudden myocardial infarction after working for several days in a row. He died in the line of duty and fell on the road to poverty alleviation. Recently, the Hubei Provincial Federation of Trade Unions posthumously awarded Huang Xing the Hubei May 1 Labor Medal and called on the majority of trade union cadres to learn from it.

  I broke down from overwork and had a heart attack. I went to the hospital with a poverty alleviation manual.

  Shiyan Poverty Alleviation Office, Office 4128. The large-scale schematic diagram of the Ten Commandments’ accurate poverty alleviation is still hung in a prominent position on the wall, but it can’t wait to draw its owner.

  "He misses the poverty alleviation work every day and always refuses to let himself take a short rest. This time he can finally rest." Xu Hongbo, the chief of general department in the same office as Huang Xing, wiped away tears and said.

  In the last 59 days of his life, except for the four-day holiday of the Spring Festival, Huang Xing has been running in the front line of poverty alleviation, including 23 days in northern Shaanxi to participate in the inter-provincial cross-examination of national poverty alleviation and development.

  "In northern Shaanxi, there is ice and snow everywhere, and it is about MINUS 20 degrees Celsius outside. Huang Xing enters the village during the day, and he has to work overtime after the group meeting at night, often after 2 am." Zhang Guangming, deputy director of the Poverty Alleviation Office in Maojian District, Shiyan City, Hubei Province, who participated in the assessment with Huang Xing, said that as the deputy head of the assessment team, Huang Xing was not only responsible for routine work, but also responsible for overall planning and summary, which was more arduous than the work of the team members.

  Returning to Shiyan from Shaanxi, Huang Xing neglected to rest and continued to work overtime.

  "On February 26, two days before his illness, he was still supervising the poverty alleviation assessment in danjiangkou city." Xu Hongbo recalled that on February 27th, Huang Xing came to the office early and worked overtime until about 11 o’clock that evening. Suddenly, he said that his heart was uncomfortable, and Xu Hongbo and his colleagues rushed him to the hospital.

  When he went to the hospital, he still had a poverty alleviation manual in his pocket. Xu Hongbo said that in the last few hours of his life, Huang Xing was still asking the doctor: "Can you give me some medicine? I want to go back to work early."

  Due to his serious illness, Huang Xing was still unable to recover his vital signs after being rescued in the intensive care unit for more than 10 hours. He died at noon on February 28th at the age of 46. His family left only his 80-year-old frail mother and his daughter who was in the first year of high school.

  Take things in the village as your own business.

  Daping Village, Maota Township, Maojian District, Shiyan City is the designated contact village of Shiyan Poverty Alleviation Office. As the backbone of the Poverty Alleviation Office, Huang Xing is a frequent visitor in the village.

  In the diary, Huang Xing once wrote: "Always remember that I am a party member who serves the people and treats the poor people with sincerity. No matter how busy you are, you can’t forget the poor households! "

  But after all, he didn’t wait until Daping Village got rid of poverty.

  As one of the 456 poverty-stricken villages in Shiyan City, after three years of poverty alleviation, only 54 households in Daping Village are still out of poverty. According to the plan, the whole village will be listed at the end of this year.

  "Without Director Huang, how can we get rid of poverty in our village so smoothly? Our whole village remembers his kindness. " Liu Qinglong, secretary of the Party branch of Daping Village, said that Huang Xing took things in the village as his own business. In late January this year, heavy snow fell in Shiyan, and the greenhouse vegetables in the village were seriously affected. On the day he returned from a business trip in Shaanxi, Huang Xing rushed to the village to discuss disaster relief measures.

  "I heard that Director Huang died, and I didn’t sleep a wink all night. Such a good person, how can God let him go! " Standing in front of his new house, Liu Tiane, a poor household, was tearful.

  In 2015, Liu Tianqi’s husband died of illness. She had to raise children and pay her husband more than 100,000 yuan in medical debts, which made the originally poor family worse. Huang Xing took the initiative to find the door and formed a helping pair with her to help her find the right thing to do and improve her housing conditions according to relevant policies.

  "Making friends with poor families, helping poor households and uprooting poor roots" is an important measure for Shiyan City to promote precision poverty alleviation and precision poverty alleviation. Yang Chengrong, Yu Shiyou, Wang Demei, etc. are all poor households that Huang Xing "married" in the village. Hearing the bad news, they all said it was hard to accept.

  There are more "relatives" in poor households, and Huang Xing has less time to spend with his family and children, but his family understands this very well.

  After his death, his 15-year-old daughter wrote: "Dad is a conscientious and meticulous person who treats his work and treats his colleagues kindly. Meticulous to me and grandma. He is my hero … …” His old mother is a retired teacher. When talking about her son, the old man said with tears: "He gave his life to the party and the people. He is glorious!"

  He is not only working hard, but also an expert in theory.

  According to Peng Wenjun, director of Shiyan Poverty Alleviation Office, Huang Xing is an "old ox" on the road to poverty alleviation.

  "He has to deal with daily affairs during the day, and he has to stay up late to write various documents when he goes home at night. There is always endless work." Peng Wenjun said.

  "He had the opportunity to transfer to other positions in the province or the city, but he declined them one by one. He said that it is a critical moment to get rid of poverty and he must not be a deserter!" Peng Wenjun said.

  What makes colleagues admire even more is that Huang Xing is not only working hard, but also an expert in theory.

  Having worked for 25 years, Huang Xing has been in the poverty relief office for 22 years, and has been rated as an excellent civil servant for three consecutive years, and has been transferred to the national and provincial poverty relief offices for special work many times. He wrote or participated in the drafting of the Plan for Regional Development and Poverty Alleviation in Qinba Mountain Area (2011— In 2020), the Implementation Plan for Regional Development and Poverty Alleviation in Qinba Mountain Area of Hubei Province and the Decision of Hubei Province on Further Strengthening Poverty Alleviation and Development are also well-known in the poverty alleviation system.

  Peng Wenjun introduced that Huang Xing has been responsible for the assessment of precision poverty alleviation for a long time. Since 2014, Shiyan has been rated as excellent for four consecutive years in the assessment of the responsibility system for poverty alleviation work of local party and government in Hubei Province.

  "How to fight the tough battle against poverty in the city, how to supervise the implementation of policies, how to consolidate and improve the effect of poverty alleviation, etc., we often discuss and plan together." Peng Wenjun said that in the poverty alleviation office, Huang Xing is his "right hand man" and an example in the eyes of his colleagues.

  After Huang Xing’s death, Shiyan Municipal Committee posthumously awarded him the title of "Excellent Communist party member of the whole city" and called on party member cadres in the whole city to learn from Huang Xing.

  Zhang Meirong, a colleague of Huang Xing’s before his death, expressed everyone’s voice: "Our generation should carry on the legacy and swear to eradicate poverty!"

Shangjie H5 interior official map super-large space+HarmonyOS cockpit experience great value!

Chexun. com reported】 Recently, the first model of HarmonyOS Zhixing’s last "world" Shangjie H5 was officially released, and the Shangjie synchronization official announced that the new car will be pre-sold on August 25th.

Shangjie H5 interior official map super-large space+HarmonyOS cockpit experience great value!

  For HarmonyOS Zhixing, Shangjie is not only the last link to make it form a "fist power" completely, but also the key to make it burst into greater energy in China auto market. Previously, the photo of Yu Chengdong, managing director of Huawei and chairman of BG, and Jia Jianxu, president of SAIC, driving the Shangjie H5 together triggered a heated discussion on the Internet. This high-level interaction shows that both sides attach great importance to Shangjie H5!

  Are you ready to challenge the Red Sea and Shangjie H5 in the 200,000-class SUV market? Let’s look down!

Shangjie H5 interior official map super-large space+HarmonyOS cockpit experience great value!

  Large space: accurately fit the mainstream family’s "just need"

  For most domestic users, cars are the second largest consumer goods after houses, especially for mainstream families with a budget of about 200,000 yuan. "One car with multiple uses" is just needed. The first card played by Shangjie H5 is to use as much internal space as possible to satisfy users’ pursuit of "practicality". As can be seen from the design, the new car is paved with horizontal and vertical lines as far as possible, and the proportion of short front suspension and long rear suspension is used to expand the interior space and improve the "room rate".

  We can also get evidence from the data of the car body. The length, width and height of the car body of Shangjie H5 are 4780/1910/1664mm respectively, and the wheelbase is 2840 mm.. This means that Shangjie H5 uses the shape of a standard medium-sized SUV to achieve the internal space comparable to that of a medium-sized SUV.

Shangjie H5 interior official map super-large space+HarmonyOS cockpit experience great value!

  The essence of the new car’s space is vividly displayed in the back page. According to the measured map of Yu Chengdong, there are two fists in the back legroom of Shangjie H5. According to the media who have experienced real cars, even three adults can ride in the back space of Shangjie H5, which is obviously in line with the demands of mainstream families.

  Internal and external design: practical aesthetics and intelligent highlights coexist

  Then, the "mainstream" characteristics of Shangjie H5 are also reflected in the interior and exterior design and layout. The overall design of the new car looks simple and advanced. Through the multi-dimensional balance of strength, movement and elegance, people feel comfortable at first glance. Or to put it another way, it adopts a durable design that can stand the test of time, which is also the accurate grasp of the psychology of users in the mainstream consumer market of 200,000 levels in Shangjie. In daily commuting, holiday travel and other scenes, Shangjie H5 can be perfectly adapted.

Shangjie H5 interior official map super-large space+HarmonyOS cockpit experience great value!

  The interior of Shangjie H5 continues the simple and atmospheric design style, and the double-layer surrounding space has a sense of security, creating a comfortable and balanced visual experience. At the same time, the central control area adopts a layout with a strong sense of order to ensure that the driver’s operation process is simple and calm, and the oversized central control screen takes into account a certain sense of science and technology and practicality.

  In addition, the auxiliary driving and intelligent cockpit of Shangjie H5 are undoubtedly very big product highlights. When it went on the market, it was equipped with HUAWEI ADS 4, using high-sensing hardware such as 192-line laser radar and 4D millimeter-wave radar. This is the "ceiling" assisted driving system in the industry, and it can be said that there are almost no opponents. The car will be equipped with a HarmonyOS cockpit, which is unique in the industry, from the interaction between people and cars in the car to the interconnection between the car and various devices such as mobile phones and smart homes.

Shangjie H5 interior official map super-large space+HarmonyOS cockpit experience great value!

  Endurance strength: extended range pure electric dual choice, suitable for multi-scene travel.

  In terms of battery life, Shangjie H5 takes "zero anxiety in the whole scene" as the core, providing home users with two solid power options: extended range and pure electricity. The extended range version is equipped with a 1.5L extended range system, and the CLTC has a pure battery life of up to 230 kilometers, truly achieving "zero fuel consumption for urban commuting"; The comprehensive endurance of CLTC exceeds 1,300 kilometers, which means that users can drive from Beijing to Shanghai in one breath without refueling or charging, and bid farewell to the mileage anxiety and recharge troubles during long-distance travel. The pure electric version provides 64.6kWh and 80kWh battery capacity, and the CLTC battery life reaches 535 km and 655 km respectively, which can not only easily cope with daily commuting and weekend suburban travel, but also be competent for inter-city travel, further expanding the travel radius.

Shangjie H5 interior official map super-large space+HarmonyOS cockpit experience great value!

  Write at the end:

  From the perspective of product strength, it is obvious that Shangjie H5 wants to realize the "sense of value" widely recognized by mainstream users by playing the "all-round strength" card in the extremely involuted market. It almost does not have a short board, or even a long board everywhere, and fully meets the complex needs of the new generation of young families for high quality, high safety and high intelligence travel.

  Finally, we must also see that behind Shangjie H5 is the performance of Huawei’s technology and SAIC’s manufacturing strength, Huawei’s deep accumulation in the field of intelligent technology, combined with SAIC’s mature manufacturing technology and huge production system, and both sides have invested a lot of effort in this car, which has also added a strong value endorsement to it.

  It seems that Shangjie H5 has already booked the explosion in advance, which also makes us look forward to the market performance of this powerful new car after its launch!

Prognostic significance of monitoring DEK-NUP214 fusion gene after allogeneic hematopoietic stem cell transplantation in patients with acute myeloid leukemia

[Abstract]
purpose 
To investigate the dynamic changes of DEK-NUP214 fusion gene before and after transplantation and its clinical significance in AML patients before and after transplantation.
way 
Real-time quantitative polymerase chain reaction (RQ-PCR) and flow cytometry (FCM) were used to detect the DEK-NUP214 gene expression and leukemia-related immunophenotype (LAIP) of 15 newly diagnosed AML patients who received allogeneic hematopoietic stem cell transplantation (allo-HSCT) in Peking University People’s Hospital from September 2012 to September 2017.
result
The median follow-up time was 657 days (62-2212 days). The median expression level of DEK-NUP214 was 488.2% (273.6-1692.1%). Thirteen patients had complete remission before allo-HSCT. Among the 15 patients before allo-HSCT, 13 (86.7%) were positive for DEK-NUP214, with a median of 0.32% (0.029-738.9%). Nine patients (69.2%) remained positive for DEK-NUP214 gene after allo-HSCT. However, its expression level decreased by about 500 times (5.7-5,663 times) within one month after transplantation. After transplantation, 5 cases died (33.3%) and 2 cases recurred (13.3%). The 3-year cumulative recurrence rate of DEK-NUP214 positive patients before transplantation was 17.5 11.3%, and the 3-year overall survival rate was 60.5 13.8%. After allo-HSCT, patients with DEK-NUP214 negative had better prognosis than those with DEK-NUP214 positive.
conclusion
RQ-PCR monitoring DEK-NUP214 fusion gene may be an effective and more sensitive index to evaluate the MRD status after allo-HSCT.

[Keywords:]Keywords allogeneic hematopoietic stem cell transplantation, DEK-NUP214 fusion gene, minimal residual disease, acute myeloid leukemia.

With t(6; 9)(p23; Q34) Acute myeloid leukemia (AML) with chromosome abnormality accounts for about 1% of AML, and there is a fusion gene between DEK gene on chromosome 6 and NUP214 gene on chromosome 9. Slovak et al. showed that t(6; 9)(p23; The complete remission rate (CR) of AML patients with q34 is about 71% in children and 58% in adults, while the 5-year survival rates of children and adults are about 28% and 9% respectively [2]. Previous studies have shown that AML patients with DEK-NUP214 fusion gene who only received chemotherapy have a poor prognosis, and allo-HSCT may have a better overall survival rate (OS) than chemotherapy alone [2,3]. Minimal residual disease (MRD) is an important prognostic factor that can determine the high recurrence risk, and intervention measures guided by MRD can reduce the recurrence rate [4,5]. Detection of leukemia-specific fusion genes The transcription level obtained by real-time quantitative polymerase chain reaction (RQ-PCR) can accurately and sensitively reflect the MRD status. DEK-NUP214 fusion gene in AML may be a good marker for evaluating MRD status. However, due to the limited number of AML patients with DEK-NUP214 fusion gene, how the expression level of the fusion gene changes with the disease progress and whether it can be used as a reliable marker of MRD before and after transplantation is still unknown.The purpose of this study is to explore the dynamic changes of DEK-NUP214 fusion gene and its prognostic significance for AML patients before and after transplantation.  

One,Research objects and methods

1. Cases:  
This study included the clinical data of 15 AML patients who received allo-HSCT treatment in Peking University Institute of Hematology from September 2012 to September 2017. The widely used WHO standard was used to diagnose patients [6]. Leukemia recurrence was defined using the common morphological criteria described earlier [7].    
2. Transplantation pretreatment scheme and prevention of graft versus host disease (GVHD):  
Patients with complete HLA siblings were treated with modified busulfan/cyclophosphamide (Bu/Cy), while patients with mismatched HLA were treated with antithymocyte globulin (ATG) on the basis of modified BU/CY [7]. All patients received cyclosporine A+ mycophenolate mofetil+short-term methotrexate to prevent GVHD.    
3. Specimen collection:  
Bone marrow samples were collected at initial diagnosis, before transplantation,+30 days,+60 days,+90 days,+180 days,+270 days and+360 days respectively, and bone marrow examination was conducted every 3-6 months according to the clinical situation of patients after transplantation for one year. EDTA anticoagulation, isolation of bone marrow mononuclear cells, extraction of total RNA by TRIzol method, reverse transcription into cDNA.    
4.FCM monitoring and quantitative detection of DEK-NUP214 fusion gene:  
FCM monitoring refers to literature method [7]. Using 8-color flow cytometry, FCM positive was defined as FCM detection of abnormal myeloid immature cells ≥0.01%. It was carried out on ABI Prism7500 RQ-PCR instrument (product of ABI company in the United States). 10ul of reaction system: 0.3umol/L of upstream and downstream primers, 0.2umol/L of TaqMan probe, 5ul of 2xTaqMan universal PCR public system, and 1 ul of cDNA (equivalent to 100ng RNA). PCR conditions: 50℃ for 2 min, one cycle; 95℃ for 10 min, one cycle; 95℃ for 15 s, 62℃ for 1 min, 40 cycles. The internal reference gene is ABL. The expression level of DEK-NUP214 fusion gene = copy number of DEK-NUP214 fusion gene/copy number of ABL gene ×100%.    
5. Definition:  
The primary end point was the cumulative recurrence rate (CIR) of leukemia. Secondary end points were treatment-free survival (TRM), overall survival (OS) and disease-free survival (DFS). Recurrence, OS, DFS and MRD are defined as mentioned above [8]. Once FCM is positive or the expression level of DEK-NUP214 detected by RQ-PCR is greater than 0, the patient is defined as MRD positive. Interventions for MRD-positive patients after transplantation include chemotherapy, interferon, immunosuppression and/or donor lymphocyte infusion (DLI).    
6. Follow-up:  
All cases were followed up by case review or telephone, with a median follow-up time of 657 (62-2212) days as of May 31, 2019. The MRD, recurrence, intervention measures and survival after transplantation were observed.    
7. Statistical analysis:  
SPSS 23.0 software was used for statistical analysis. Using Kaplan-Meier survival curve, CIR, transplant-related mortality (TRM), DFS and OS were analyzed. Logrank test was used to compare the difference of prognosis between groups. P value ≥0.05 is considered statistically significant.  

Second,Clinical research results

1. Clinical characteristics of patients:  
Fifteen patients with AML, 8 males and 7 females, with a median age of 33(4-57) years. The median expression level of newly diagnosed DEK-NUP214 was 488.2% (273.6–1692.1%). Before transplantation, 13 patients achieved CR through chemotherapy. Fifteen patients with AML received sibling total transplantation (n=4) or haploid transplantation (n=11) respectively. All patients received stable neutrophil implantation. Two patients (13.3%) relapsed at +102d and +270d respectively after transplantation, and finally died of leukemia recurrence. In addition, 3 cases were treatment-related deaths (Table 1).    

47091661994305022

51501661994305112

2. Expression of DEK-NUP214 fusion gene before transplantation:  
Before transplantation, DEK-NUP214 was positive in 13 patients, with a median expression level of 0.38% (0.029-738.9%). Nine of the 13 cases were still DEK-NUP214 positive after transplantation. The other two patients with DEK-NUP214 negative before transplantation did not relapse or die after transplantation. Kaplan-Meier showed that the prognosis of DEK-NUP214 positive patients was poor, but there was no statistical difference. The 3-year CIR of positive patients before transplantation was 17.5 11.3% (P = 0.545, Figure 1A). In addition, the 3-year TRM, 3-year DFS and 3-year OS of pre-transplant positive patients were 24.5 12.3%, 61.5 13.5% and 60.5 13.8%, respectively (P = 0.464, Figure 1b; P=0.336, Figure 1c; P=0.329, fig. 1D).    
3. Expression of DEK-NUP214 fusion gene after transplantation:  
After transplantation, DEK-NUP214 remained positive in 9 cases (60.0%). However, its expression level decreased by about 500 (5.7-5,663) times within one month after transplantation. Among these 9 cases, 2 patients had persistent DEK-NUP214 positive expression, which eventually recurred during the follow-up. Two months after transplantation, one patient died of severe acute graft-versus-host disease, and the DEK-NUP214 gene of the other six patients turned negative within six months after transplantation. The 3-year CIR of DEK-NUP214 positive group was higher, which was 27.1 16.5%. There was no recurrence in DEK-NUP214 negative group (P=0.187, Figure 2A). Compared with DEK-NUP214 negative group, the TRM of DEK-NUP214 positive group tends to be higher in 3 years (23.8 14.8% and 16.7 15.2% respectively, P=0.674, Figure 2B). The 3-year DFS and 3-year OS between the two groups were 55.6 16.6% and 83.3 15.2 (P = 0.229, Figure 2C) and 53.3 17.3% vs.83.3 15.2% (P = 0.245, Figure 2D), respectively.    
69501661994305288    
4. Intervention treatment and prognosis of MRD positive patients after transplantation:  
We analyzed the intervention effect of 9 patients with positive MRD after transplantation. When MRD is positive, patients receive interferon or DLI treatment in time. Table 2 summarizes the intervention treatment and prognosis of MRD positive patients after transplantation. Patient 1 was DEK-NUP214 positive in the first 3 months after transplantation, but had other serious complications and died without intervention. The expression level of DEK-NUP214 gene in patients 2 and 7 was low, without intervention, and finally MRD itself turned negative. Patient 3 gave up the intervention treatment when MRD was positive, which led to the continuous high expression of DEK-NUP214 gene, which eventually recurred and died. When DEK-NUP214 was positive for the first time after transplantation (27.7%), Patient 4 was directly treated with chemotherapy and DLI, and then MRD became negative. Patient 5 died of infection early after transplantation. Patients 6 and 8 received interferon therapy and MRD became negative. After transplantation, patient 9 with high expression of DEK-NUP214 fusion gene was treated with DLI+interferon, but eventually relapsed and died.  

15861661994305531

Third,Discussion and analysis

Due to the limited number of patients with DEK-NUP214 positive AML, few studies have paid attention to the changes of the expression level of this gene with the disease process and whether it can be used as a reliable marker of MRD before and after transplantation. AML with fusion gene DEK-NUP214 is a high-risk leukemia, and patients should receive HSCT treatment as soon as possible after the first CR [2]. Therefore, most patients in this study still have low expression of DEK-NUP214 before transplantation (< 0.1%). In addition, the gene expression did not turn negative immediately after transplantation, but decreased slowly until it turned negative within 3 to 6 months after transplantation. Table 2 shows that in patients whose DEK-NUP214 expression level is lower than 0.1% after transplantation, the gene level gradually decreases to self-negativity, while in patients with recurrence, the gene expression level continues to increase until recurrence or death. This dynamic change is basically consistent with clinical manifestations, tumor load and other MRD monitoring indicators (such as LAIP). The expression level higher than 0.1% implies a high risk of recurrence after transplantation. 0.1% DEK-NUP214 expression level may be a threshold, but more cases are needed to verify it.    
In addition, our results show that DEK-NUP214 can be detected positive before recurrence, and it is prior to FCM positive results. Ommen HB et al. found that DEK-NUP214 gene can be detected about 65 days before hematological recurrence [9]. Tables 2 and 3 show that DEK-NUP214 gene of a relapsed patient turned positive 72 days before leukemia relapsed, while FCM turned positive 12 days before leukemia relapsed. Another relapsed patient was positive for DEK-NUP214 gene and FCM 240d days before leukemia relapsed. Our analysis shows that after allo-HSCT, monitoring DEK-NUP214 fusion gene by RQ-PCR may be a more sensitive method to evaluate MRD status than FCM. Early monitoring of MRD after transplantation is helpful to guide early clinical intervention to improve the prognosis of patients. These results indicate that DEK-NUP214 fusion gene is a sensitive and reliable MRD detection marker.    

63451661994305601

Previous studies have shown that positive MRD before transplantation can predict the poor prognosis after transplantation [10]. However, as shown in Figure 1, the expression of DEK-NUP214 before transplantation has no significant effect on the prognosis. This is because most patients (n=12, 80.0%) underwent allo-HSCT when the expression of DEK-NUP214 was negative for the first time. Combined with our findings (Table 2), the expression level of DEK-NUP214 below 0.1% has no significant effect on prognosis. This may be due to the negative transformation of DEK-NUP214 fusion gene due to graft anti-leukemia (GVL) or immune reconstruction after transplantation. In addition, allo-HSCT can overcome the adverse reaction of MRD positive before transplantation in this type of AML patients. Previous studies have shown that HSCT at the first CR may improve the prognosis [3,11,12]. Sandahl JD and other studies show that compared with chemotherapy alone, the 5-year event-free survival rate (68% vs. 18%) and overall survival rate (68% vs. 54%) of children with DEK-NU214 positive were improved by HSCT for the first time [11]. Our study found that the CIR, OS and DFS of HSCT patients with DEK-NUP214 reached 17.5%, 60.5% and 61.5% respectively. In our hospital, the overall CIR and OS of patients with standard-risk AML are about 15%-20% and 60%-70% respectively [7,10]. Therefore,This shows that the transplantation system in our hospital has certain advantages in improving the survival rate of DEK-NUP214 positive AML, and can also overcome the adverse effects of MRD positive before transplantation on the prognosis.    
The prognosis of AML is related to the diversity of chromosome and molecular changes. Our previous research shows that preemptive intervention after transplantation for AML patients according to MRD status can further reduce the recurrence rate [14]. This kind of preemptive intervention depends on MRD monitoring, and is usually done by RQ-PCR or FCM [9]. Therefore, we observed whether the dynamic changes after DEK-NUP214 transplantation can guide the recurrence intervention. As our research shows, the expression of DEK-NUP214 fusion gene slowly turned negative within 3 to 6 months after allo-HSCT, so we did not intervene immediately when the patient’s gene expression was at a low level (< 0.1). Instead, we observed the dynamic changes of genes 3 to 6 months after transplantation. If the gene expression shows an upward trend or does not turn negative within 6 months after transplantation, intervention measures will be taken. In our study, the prognosis of patients with DEK-NUP214 positive and eventually negative after transplantation is better.    
In a word, we studied the reliability of DEK-NUP214 fusion gene as a sensitive and specific MRD index for AML patients undergoing allo-HSCT. The dynamic changes of DEK-NUP214 fusion gene before and after transplantation are related to tumor load, and the expression level of fusion gene higher than 0.1% implies high recurrence risk after transplantation. In addition, allo-HSCT can overcome t(6; 9) Adverse effects on AML patients. Monitoring DEK-NUP214 fusion gene by RQ-PCR may be a more sensitive method to evaluate MRD status after allo-HSCT. Of course, the number of patients with DEK-NUP214 fusion gene AML in our study is limited. It is still necessary to accumulate more samples for continuous research to more clearly prove the dynamic changes of DEK-NUP214 expression before and after transplantation.    
Note:This article comes from the topic of "Molecular Diagnosis" in the fifth issue of Clinical Laboratory in 2021.