At the 2020 China Biotechnology Innovation Conference, experts pointed out that stem cells, as strategic resources, are strategic, public-spirited and supportive, in which the strategy is for national security and future competition, the public-spirited is for population health and social development, and the supportive is for scientific, technological, industrial and economic development. It is an important measure to ensure people’s health to collect, store, develop, utilize and share strategic stem cell resources and promote clinical transformation.

Stem cell resources are important strategic resources of the country. How to safely and effectively preserve China’s special, unique and rare human biological resources is of great significance for promoting China’s population health, maintaining the safety of life resources and treating major diseases.

One of the important values of stem cells is their application in disease treatment.

Professor Thomas C. Sudhof, winner of the Nobel Prize in Physiology or Medicine, once said, "We can control the disease and stem cells are a very good treatment. " Stem cell research will bring new hope to the problems that traditional medical treatment can’t solve.. "

Internationally, the United States, South Korea, Japan, the European Union and so on have approved stem cell therapy products listed, and stem cell therapy for diseases is becoming a more extensive reality.

By April, 2021, more than 1,200 clinical trials of mesenchymal stem cells were retrieved from ClinicalTrials.gov website.

In recent years, this number has continued to increase, indicating that the importance of mesenchymal stem cells is increasing.

At present, the research and development of stem cell drugs in China is becoming more and more standardized, attracting a lot of capital and talents. At the moment when the results are about to blow out, we look forward to this market by combing the foreign mesenchymal stem cell drugs.

According to incomplete statistics, there are 18 mesenchymal stem cell products that have been approved for marketing in the world, and 10 products meet the definition of drugs.

18 products related to mesenchymal stem cells (including orphan drugs, conditionally approved drugs, medical products, etc.); The date and country of approval are the first approval; Multistem is not counted, because its official website claims that the drug is not mesenchymal stem cells.

01

More flowers, Mesoblast

Prochymal: graft versus host disease

Country: USA, Canada, New Zealand

Date: May 2010

Cell type: bone marrow mesenchymal stem cells

Indications: graft versus host disease, Crohn’s disease

Price: $200,000/course of treatment

Initially, the FDA of the United States approved it as an orphan drug for the treatment of type I.

In 2012, Prochymal was sold to Mesoblast in Australia, and was approved for marketing in Canada and New Zealand to treat acute graft-versus-host disease.

In 2015, Mesoblast cooperated with JCR and renamed it Temcell to be listed in Japan.

Temcell: graft versus host disease

Country: Japan

Date: February 2016

Cell type: bone marrow mesenchymal stem cells

Indications: graft versus host disease

Price: about $120,000/course of treatment.

As mentioned above, Temcell was formerly Prochymal of Osiris Company and Ryoncil of Mesoblast Company.

In 2019, TEMCELL was approved to treat epidermolysis bullosa and neonatal hypoxic-ischemic encephalopathy.

In JCR’s financial report, Temcell’s sales revenue was about 18 million US dollars in 2018 and 28 million US dollars in 2019.

MPC: bone repair

Country: Australia

Date: July 2010

Cell type: autologous mesenchymal precursor cells

Indications: bone repair

Price: unknown

In 2010, the MPC(Mesenchymal Precursor Cell) independently developed by Mesoblast was approved for listing in Australia for the repair and regeneration of damaged tissues.

Mesoblast’s current market value is about $1 billion, and its business income of $6.6 million in fiscal year 2020 comes entirely from Temcell, mainly from milestone payments ($25 million) from China, Japanese and other partners.

02

Try first, Korea FDA

CellGram: acute myocardial infarction

Country: Korea

Date: July 2011

Cell type: autologous bone marrow mesenchymal stem cells

Indications: Acute myocardial infarction

Price: $15,000

CellGram was used by coronary angioplasty within 72 hours after chest colic to improve the left ventricular blood cell count in patients with myocardial infarction. After 6 months of treatment, the measurement showed that each treatment improved the left ventricular ejection fraction by 6%.

By 2017, this therapy has created sales of about $7.8 million. However, CellGram failed to meet the requirements of treating 600 patients within a six-year period, and Pharmicell applied to reduce the minimum number of patients to 60, which was rejected.

Some industry observers speculate that South Korea will cancel its approval, but this is the first stem cell drug in South Korea. "The cancellation of approval will have a harmful impact on the national reputation, and we must make a cautious decision."

Cartistem: degenerative arthritis

Country: Korea

Date: January 2012

Cell type: umbilical cord blood mesenchymal stem cells

Indications: Degenerative arthritis and knee cartilage injury.

Price: 19,000-21,000 USD

In the eight years since Cartistem was put on the market, the cumulative sales have exceeded 18,000 doses.

From 2017 to 2020, Cartistem has sold more than 10 billion won (about 9 million US dollars) for four consecutive years, which is obviously better than the other three stem cell drugs approved by South Korea (the annual sales are less than 5 billion won).

Medipost quarterly sales trend (millions of won, source: Medipost)

Researchers such as Kim SJ conducted a retrospective analysis of 128 product users, and found that after two years of follow-up, the pain and function of these patients were improved, which was statistically significant.

At present, the product has completed phase I and IIa clinical trials in the United States, and phase II clinical trials are being carried out in Japan.

Cupistem: Crohn’s disease

Country: Korea

Date: January 2012

Cell type: autologous adipose-derived mesenchymal stem cells

Indications: complex Crohn’s disease complicated with anal fistula

Price: 3000-5000 USD

Cupistem is used to reduce inflammation of Crohn’s fistula.

In a phase II clinical trial, 43 patients were evaluated for fistula healing after 12 months, and 80.8% of them showed complete fistula healing. After 24 months, 75% of these patients were completely closed, showing sustainable safety and therapeutic effect.

NeuroNATA-R: amyotrophic lateral sclerosis

Country: Korea

Date: July 2014

Cell type: autologous bone marrow mesenchymal stem cells

Indications: amyotrophic lateral sclerosis, motor neuron disease

Price: USD 18,000-72,000 (according to weight)

NeuroNATA-R is a stem cell therapy provided by Corestem Company of South Korea. Mesenchymal stem cells need to be collected from the patient’s own bone marrow four weeks before each injection of stem cells.

In the phase I clinical trial to evaluate the safety, 7 patients received intrathecal injection twice and were followed up for 12 months. The research shows that it has good security.

03

Not to be outdone, Japan and India

Stempeucel: Severe limb ischemia

Country: India, ratified by EU.

Date: June 2015

Cell type: bone marrow mesenchymal stem cells

Indications: thromboangiitis obliterans

Price: $2,200 (2017)

In 2015, Stempeucel was approved by the European Medicines Agency (EMA) as an orphan drug to treat thromboangiitis obliterans.

In 2017, the General Administration of Drug Administration of India (DCGI) granted Stempeucel a limited marketing license to treat patients with severe limb ischemia caused by Buerger’s disease. In 2020, DCGI completed the formal approval.

The research and development of Stempeucel lasted for 12 years and finally became the first allogeneic cell therapy product approved for commercial use in India.

Alofisel: Crohn’s disease

Country: Japan, EU ratification

Date: March 2018

Cell type: adipose-derived mesenchymal stem cells

Indications: complex perianal fistula in patients with Crohn’s disease

Price: about $70,000/course.

In 2018, Takeda, a Japanese pharmaceutical giant, and TiGenix, a Belgian stem cell company, jointly announced that EMA had approved Alofisel (CX 601) for the treatment of Crohn’s disease complicated perianal fistula whose fistula failed to respond to at least one traditional or biological therapy.

However, Alofisel encountered some difficulties in obtaining the so-called third-level approval, which is a term used to refer to national payer insurance. For example, NICE in the UK refused to recommend Alofisel on the grounds that it did not benefit much from clinical trials.

In 2019, Takeda decided to adopt value-based pricing in Europe. In other words, if patients do not improve, they will bear part or all of the expenses.

In the fiscal year ending March 31st, 2020, Takeda earned about $350,000 from Alofisel.

Stemirac: spinal cord injury

Country: Japan

Date: 2018-12

Cell type: autologous bone marrow mesenchymal stem cells

Indications: spinal cord injury

Price: $135,000

The listing of Stemirac is somewhat controversial. It only did phase II clinical trials, and it was conditionally approved and included in the national health insurance.

This move was criticized by Nature, who thought that there was no control group in its clinical trial, and there were only 13 subjects in the treatment group. The Japanese side responded that setting up a control group may cause moral problems.

According to an article in 2019, Stemirac will bring about $3.4 million in revenue to Nipro every year in the next nine years.

04

The pace of clinical treatment of stem cells in China is accelerating!

According to the data of National Medical Products Administration Drug Evaluation Center, up to now, eight new drugs of mesenchymal stem cells have been applied for clinical approval in China, and the indications are acute graft-versus-host disease, rheumatoid arthritis, ischemic stroke, knee osteoarthritis and diabetic foot.

Source: CDE

At present, the supervision of stem cell therapy in China adopts a "dual-track management model". National Health Commission mainly manages clinical research conducted by medical institutions, and the Food and Drug Administration is mainly responsible for stem cell therapy products that are applied for marketing after clinical trials are completed according to drugs.

In recent two years, the clinical application of stem cells in China has been standardized under the "dual-track management model".Up to now, 74 stem cell clinical research projects have passed the record of National Health Commission. Eight applications for new stem cell drugs have been approved by the Food and Drug Administration..

Compared with the past few years, the clinical transformation of stem cells in China is developing with a brand-new trend, and the blank of domestic stem cell listed drugs is expected to be filled faster.

At present, in the field of stem cell transformation, the research in China mainly focuses on stem cell bank, stem cell drug application, preclinical research and clinical trials. The gradual improvement of regulatory policies has pushed the clinical transformation of stem cells in China to a new development situation.

First, China has entered a new stage of development of new stem cell drugs.The promulgation of "Technical Guiding Principles for Research and Evaluation of Cell Therapy Products (Trial)" clarified the standard of stem cell therapy as a drug declaration, and at the same time, the new drug approval system of "60-day clinical trial implied permission" provided new development opportunities for the research and development and declaration of new stem cell drugs in China.

Second, the national "stem cell and transformation research" focuses on promoting the development of stem cell therapy drugs.For example, one of the assessment objectives pointed out in the key special declaration guidelines is to develop cell therapy drugs for a major or rare disease, apply for registration of new stem cell drugs and apply for clinical approval of stem cells.

Third, the policy encourages the strength of comprehensive enterprises to promote the clinical transformation of stem cells.In the past two years, some domestic policies explicitly support qualified 3A hospitals to cooperate with qualified social forces to declare and carry out stem cell clinical research, that is, to support and encourage qualified social forces or preparation institutions (enterprises) to join the stem cell industry and jointly promote the clinical application and development of stem cells. The promotion of enterprise innovation forces has accelerated the development and application of stem cells in China.

At present, there are still many diseases that need innovative therapies to solve the unmet clinical needs, and the expansion of indications for new stem cell drugs is precisely to meet these needs.

05

Perinatal tissue stem cells have gradually become mainstream.

Perinatal tissue is one of the largest sources of mesenchymal stem cells, and it has become a popular source of stem cells for new drug research and development. Mesenchymal stem cells derived from perinatal tissues have the advantages of multi-directional differentiation potential, immunological characteristics, convenient and harmless material collection, wide sources and easy industrial preparation, which has become a new trend of medical development. At present, more than 60% of the new stem cell drugs that have obtained clinical approval in China use mesenchymal stem cells derived from perinatal tissues.

In the treatment of severe patients in COVID-19, mesenchymal stem cells derived from perinatal tissues have also shown a good effect. Domestic research reports show that the critically ill patients in COVID-19 were transferred out of ICU after 9 days of reinfusion of mesenchymal stem cells from perinatal tissues, and most vital signs and clinical trial indicators returned to normal, and both throat swab tests were negative for Covid-19.

In recent years, immune cells derived from perinatal tissues have gradually become a research hotspot. Previously, the FDA approved the clinical trial of immune cells derived from human placental stem cells to treat COVID-19, which was developed by Celularity, a biotechnology company in New Jersey, USA.

In the early trial, as many as 86 patients with symptomatic COVID-19 will be treated to evaluate the safety of the treatment and the ability to prevent patients from getting worse.

Internationally, placental cell therapy products are also accelerating the pace of entering the market. For example, placenta-derived adherent cell PLX-PAD, which has attracted much attention in recent years, is being designed to treat patients who do not need tissue or genetic matching.

This therapy has passed the accelerated approval routes in the United States and the European Union, including the quick approval qualification of the US FDA and the Adaptive Pathways program of the EU EMA, for the treatment of limb ischemia. In addition, PLX-PAD has also been used in clinical research of other indications, including intermittent claudication, orthopedic indications, pulmonary hypertension and women’s health, and some of them have entered clinical phase III.

At present, China’s stem cell industry has formed a huge market scale. With the clinical transformation and market development, the era of stem cells benefiting patients in China will also come.

　　If you are annoyed that your expensive perfume doesn’t smell so good in a bottle after being sprayed on your body, or the fragrance disappears within an hour after you leave home, then there may be something wrong with the way you use perfume.

　　Francis Courgian, a famous French perfumer who has made popular perfumes of Dior, Burberry, Lancome and other famous brands, recently revealed five misunderstandings of women’s use of perfume in an interview with Vogue magazine.

　　Kurgian pointed out that to make perfume release the best taste, you must consider the environment in which you use perfume and the clothes attached to it. Not only that, if you are not used to wearing perfume every day, you’d better buy a small bottle, because the shelf life of perfume is usually only three months. As for how to use perfume correctly, let’s listen to what this perfumer has to say.

　　If you’re in the habit of spritzing your wrists and then rubbing them together, you’re actually ruining the scent.

　　If you are used to spraying perfume on your wrists and rubbing them against each other, you are actually destroying the fragrance.

　　That’s because the friction "heats up the skin, which produces natural enzymes that change the course of the scent", with the top and middle notes being worst affected as well as the dry-down – the long period during which the fragrance unfolds.

　　This is because friction "will make the skin warm, thus producing natural enzymes that will change the fragrance stage", and the former and middle tones are most affected, and the latter tone-the long process of fragrance release-will also be affected.

　　If you’re wearing a floral perfume, for instance, this heat makes it lose its crispness.

　　If you use floral perfume, the heat will make it lose its fresh taste.

　　To keep your fragrance smelling like it should, as well as making it last longer, spritz it once and then simply let it sink in.

　　In order to make your perfume give off its beautiful fragrance and make it last longer, you just need to spray it and let the perfume penetrate into your skin by itself.

　　Don’t DON’T KEEP IT IN THE BATHROOM.

　　Perfume is extremely sensitive to environmental changes and will be impacted by changes such as going from cold to hot.

　　Perfume is extremely sensitive to external changes and will be affected, such as the temperature changing from cold to hot.

　　This process sets off "unexpected chemical reactions within the natural ingredients" of the perfume such as patchouli, meaning your perfume will age faster and start to smell "off".

　　Temperature changes will trigger "unexpected chemical reactions between natural ingredients (such as patchouli)" of perfume, which means that your perfume will "age faster" and start to "taste bad".

　　The best way to store your fragrance is in its original box at room temperature.

　　The best way to store perfume is to put it in an original box and put it in a room temperature environment.

　　Dooopt for small sizes chooses perfume in small bottles.

　　If you are lucky enough to have lots of bottles or you don’t use your favourite perfume every day, oxygen will start to break down the molecules as it languishes on your dressing table.

　　If you are lucky enough to have many bottles of perfume, or if you don’t use your favorite perfume every day, then oxygen will start to break the molecular structure of perfume and make it gradually evaporate and deteriorate on your dressing table.

　　A typical bottle has a shelf life of three months so if you’re not using a perfume daily, you’re best off buying a smaller variety.

　　The shelf life of a typical perfume is three months, so if you don’t use perfume every day, you’d better buy a small bottle.

　　Or you could decant your bigger bottles into smaller containers and keep them in the fridge to maintain perfection.

　　Or you can pack your big bottle of perfume into several small bottles and put them in the refrigerator to keep the perfect fragrance.

　　DO EMBRACE SYNTHETICS chooses perfume with artificial ingredients.

　　The idea of a perfume that boasts of being all-natural might sound appealing, but the fragrance may well let you down.

　　Perfume under the banner of pure nature may sound attractive, but the fragrance of this perfume will disappoint you.

　　Musk, which was formerly derived from animals, is probably the most popular fragrance ingredient but must be created synthetically.

　　麝香很可能是最受欢迎的香水原料，过去取自于动物，现在只能人工合成。

　　Other popular smells, such as peony, freesia, and lily of the valley, can’t be extracted naturally as they don’t release enough aroma so they must be re-created.

　　其他受欢迎的香味，比如芍药、小苍兰、铃兰，不能通过自然手段提取，因为这些花释放的香味不足，所以必须人工再造。

　　According to Kurkdjian the ideal formula is a blend of essential oils, absolutes, and man-made ingredients.

　　Kurgian pointed out that the most ideal perfume formula is a mixture of essential oil, purified oil and artificial raw materials.

　　DO SPRITZ IT ON YOUR HAIR spray perfume on your hair.

　　Perfume actually doesn’t last too long on dry skin, according to Kurkdjian and as you sweat the natural oils destroy the fragrance.

　　Kurgian pointed out that perfume can’t stay on dry skin for too long. When you sweat, natural oils will destroy the fragrance.

　　You can combat this by using the matching body lotion or making sure your moisturiser isn’t scented so it doesn’t interfere.

　　You can prevent the fragrances from interfering with each other by using a body lotion that matches the scent of perfume or a body lotion without perfume.

　　If you want to ensure longevity, mist your scent lightly on your hair or on a scarf.

　　If you want perfume to last a long time, you can spray it gently on your hair or scarf.

　　’Because they move with the air, it helps with the diffusion of the scent,’ he explained.

　　He explained: "Because the hair and scarf are fluttering in the wind, it helps to diffuse the fragrance."

　　If you are putting perfume on your skin, target spots that are exposed to the air.

　　If you spray perfume on your skin, you’d better spray it on the body parts exposed to the air.

　　It’s no good putting it on pulse points such as the wrists and inner elbows if they’re covered up.

　　If the wrist or elbow is covered with clothes, it is useless to spray perfume on the pulse beating place such as the inside of the wrist or elbow.

　　(English source: Daily Mail Translation & Editor: Dany)

　　CCTV News:At 8 o’clock on the morning of December 16th, the website of the Supervision Department of the Central Commission for Discipline Inspection released a message saying that Cao Baijun, the former director of the Fourth Bureau of the International Liaison Department of the CPC Central Committee, was suspected of serious violation of discipline and was currently under organizational review.

　　　　　 Cao Baijuan was born in Nanjing, Jiangsu Province in April 1954. Graduated from Shanghai International Studies University (now Shanghai International Studies University) in 1975, majoring in German, and worked in the International Liaison Department for a long time.

　　In 2009, Shanghai International Studies University celebrated its 60th anniversary. As an alumnus, Cao Baijuan returned to Shanghai to have an exchange meeting with German teachers and students. Cao Baijuan recalled his college life. He said: "There should be three problems to be solved in college. First, study hard. Second, deal with interpersonal relationships. In addition, we should establish correct political beliefs, outlook on life and values." Referring to his life experience, he said that when he graduated from university, his goal was to become the best translator in China. To this end, he did a lot of exercises and read a lot of books. "The goal is very single, so it is easy to succeed." He described himself like this.

　　According to public information, Cao Baijun worked in the International Liaison Department of the CPC Central Committee from 1975 to 1982, and was responsible for the business of this department and German-speaking countries in Europe. In 1982, he won a scholarship from the Adenauer Foundation in Germany, and studied in the Institute of Political Science of the University of Heidelberg, Germany. The instructor was Klaus von Baimer; After returning to China in 1985, he served as the director of the German Department of the International Liaison Department of the CPC Central Committee; During this period, I participated in the reception of famous German politicians visiting China, such as johannes rau, La Fontaine, Scharping, Birna, Barr and others, and served as the chief translator. In 1992, he went to UCLA for short-term study.

　　From November 1992 to August 1993, he served as the director of the Planning Commission of Shanghai Pudong New Area Government. From August 1993 to May 1999, he served as deputy general manager and general manager of China Hualian International Trade Company; In May 1999, he served as the director of China Economic Liaison Center and concurrently served as the general manager of China Hualian International Trade Company; In 2007, he began to serve as the director of the Fourth Bureau of the International Liaison Department (Africa Bureau) until he left office.

　　After leaving office, Cao Baijuan became a company consultant. In August, 2017, a company published a news in official website that a Spanish representative came to visit and investigate, accompanied by Cao Baijuan, a senior consultant of the company. This was the news of Cao Baijuan’s last public appearance.

　　　　　 It is worth mentioning that from July 2 to August 31, 2016, the Central Fourth Inspection Team conducted a special inspection of the International Liaison Department. During the inspection, the inspection team found some problems reflected by the cadres and the masses, mainly: the "four consciousnesses" are not strong enough, there is a gap in implementing the central decision-making and deployment, and it is not enough to respond to the central concerns and take the initiative; The party building work is weakened, the ideological and political work is weak, the rectification of the party’s mass line education practice activities is not in place, and the implementation of attendance system is not strict; Strictly administering the party in an all-round way is not strong enough, supervision and discipline are loose and soft, and investigation and punishment of some obvious violations of discipline are not in place; There is not enough transparency in the selection and appointment of cadres, and there is a phenomenon of "discussing personal relationship", and the training and exchange of cadres are not enough; Spiritual behaviors that violate the eight central regulations still occur, such as office buildings, overseas delegations, official receptions, etc., and individual subordinate units illegally distribute benefits and subsidies; The management of state-owned assets and funds is lax, the management of real estate rental is chaotic, and there is a risk of clean government. At the same time, the inspection team also received reports of problems involving some leading cadres, which have been transferred to the Central Commission for Discipline Inspection, the Central Organization Department and other relevant parties for handling according to relevant regulations.

　　It is unknown whether Cao Baijun is included in the problems involving some leading cadres mentioned in the inspection report.

"Congratulations on lending 500,000 yuan, and customers can’t keep up." Xiao Bo (a pseudonym), who claimed to be an intelligent outbound service provider for telemarketing, updated his circle of friends with a screenshot of a customer’s successful lending. In Xiao Bo’s circle of friends, there are many similar "success stories". Xiao Bo claims that his telemarketing robot can make more than 1,000 calls in a single day, and the connection rate remains at 30% or even higher, and can avoid supervision and prevent titles.

Are you still bothered by harassing calls about selling loans and POS machines?

From late October to November, 2021, The Paper reporter found that the chaos in the telemarketing industry still exists. The technical model of "outbound call system+telemarketing robot" claims to be able to accurately dial the special line and mark the customer information, and evade the supervision of communication operators through technology; Behind it are hidden behaviors such as illegal trading of personal information and illegal sales of POS machines.

The so-called "anti-sealing" call-out system for electric sales

Telemarketing is a sales method relying on telephone, which contacts customers through special telephone marketing numbers to complete product promotion, quotation, transaction, etc., but this method is often easy to cause harassment to users. In the media reports, there are many cases in which telephone users are often disturbed by receiving telemarketing calls. In recent years, the Ministry of Industry and Information Technology and the three major operators have taken measures such as "high-frequency call blocking". However, with the application of intelligent voice and other technologies, the intelligent outbound calling software system, which claims to be able to make a large number of calls a day and "prevent blocking" (prevent blocking), still makes users hard to prevent.

A recent survey in The Paper found that some telemarketing software used "anti-sealing" as a gimmick to attract customers, and all kinds of "anti-sealing robots" gradually became popular in the market.

In Baidu Post Bar and Tencent QQ, the keyword "electricity sales" is searched, which shows a lot of information about "electricity sales software". The reporter randomly joined a QQ group named "Outbound Call System for Telemarketing", with more than 1,000 members and an account named "Chamber of Commerce for Telemarketing Systems" posting various promotional information related to Telemarketing from time to time in the group. "electricity sales card", "electricity sales App" and "electricity sales anti-sealing system" ……

The sales information in the group claims that the services provided can cover the whole industry such as finance, collection, loan, insurance, health care, drinks, auto insurance and games.

The reporter randomly added the WeChat of Xiao Bo, a telemarketing intelligent outbound service provider, from the QQ group. He asked the reporter what business he wanted to do, saying, "Let’s see if we can do it. Now the telecom fraud investigation is strict." After confirming that it was a "campus loan" business, he enthusiastically introduced his products and sent a price list, including a set of real-life speech made by 300 yuan, and a robot package divided into basic version, premium version and agent version according to different price points and specifications. During the conversation, Xiao Bo said that most of his clients are selling financial stocks, loan insurance and POS machine sales, all of which can be "sealed".

In his description, the "anti-sealing robot" does not need any calling cards and auxiliary equipment, and the outbound call system purchased together will use the group call line to dial the number. Regarding the reporter’s question that "making too many calls will not be seized", Xiao Bo replied that a certain line will inevitably have complaints every month, but as long as it reaches the upper limit of the complaint rate, it will stop the line and replace it with another one, so as to prevent the system purchased by customers from being blocked. He also mentioned a new business to be launched soon-an anti-sealing App for electricity sales. In the promotional picture of the App, the words "the three major operators’ Puka seconds turn white list and the high frequency does not seal the number" are placed in the most conspicuous position.

In order to further dispel the reporter’s doubts, Xiao Bo added, "We are first-class landing line operators and all cooperate with operators."

He Zheng (a pseudonym), another service provider who claimed to be engaged in the research and development of the telemarketing system for 6 years, explained to reporters that the telemarketing robot business on the market now basically adopts the "line+system+service" model. Buyers only need to use the account they provide, and they can enjoy a full set of services, "including technical support, carrying lines and after-sales."

In the 1 minute and 22 second recording of "Loan Talk" he sent, a customer on the other end of the phone in love women, who claimed to be a "customer service representative of a bank", indicated that he could handle a loan of 3-5 million yuan and "pay it back as you borrow it". In this conversation, the customer service representative repeatedly said that he was a staff member of "a bank credit center" and asked about the occupation of the telemarketing customer, the loan and insurance business he handled, and "whether there is a house or a car under his name".

He Zheng said that the so-called "bank customer service representative" bought their telemarketing system, recorded it in advance, and combined it into a complete sales talk according to the matching of the system. The customer thought it was a real person and was constantly "promoted", and might place a loan order. But in fact, they don’t know whether this "bank customer service representative" is a bank staff member or a telecom fraudster.

The "material dealer" in the dark of the electricity sales industry

In the telemarketing industry, an important part is to obtain customer information resources. The illegal trading of personal information also breeds in the dark.

After the reporter joined a QQ group, a "supplier" who claimed to specialize in health care lines (the person who provided information such as the user’s phone number) sent a message in the QQ group saying that he obtained the information of consumers who bought a health care book in TV shopping programs and then sold it to the telemarketing team or system service provider who promoted health care products. In the screenshots he sent, these consumers are distributed in different provinces such as Shandong, Henan and Zhejiang.

Compared with system service providers, "material suppliers" are more cautious. They usually don’t directly give contact information such as WeChat or telephone, and they will communicate further after confirming that the other party is an "intended customer".

In a chat with a "supplier" named AZY, the reporter learned that most customer resources are divided into "AI materials" and "hand-stripping materials". "AI material" is provided for electric sales robots, while "hand stripping material" is provided for employees who make phone calls manually. According to the feedback rate of dialing, the prices of the two "materials" are also different. Generally speaking, the feedback rate of "hand stripping" is higher than that of "AI material" and therefore the price is higher.

According to the price standard sent by AZY, 600 yuan RMB can buy 10,000 pieces of AI materials or 1,000 pieces of user information. These materials are usually replaced by different pinyin abbreviations, such as the special line for investors, which they call "GM powder", and the online loan, which is called "Wd".

There are many "anti-pit and anti-second-hand material merchants" posts in Baidu Post Bar. Some familiar "material merchants" will add WeChat to each other and set up their own WeChat groups, in order to expand or stabilize their sources of "materials".

In the investigation, the reporter found that in order to be more concealed, the transactions between suppliers and buyers often shift from WeChat to other more private online trading platforms. The line operator "AXY" chose an overseas chat software called "Telegram" to complete the transaction with the customer. After failing to reach a transaction with the reporter, "AXY" immediately closed his QQ space.

How to deal with the chaos of electricity sales

In recent years, the phenomenon of electricity sales has been repeatedly concerned and rectified.

In 2019, the central bank issued document [2019] No.85 "Notice of the People’s Bank of China on Further Strengthening the Management of Payment and Settlement to Prevent New Crimes in Telecommunication Networks", pointing to the chaos of telephone sales of POS machines. At the same time, a number of payment institutions issued a notice on strictly prohibiting online sales and electronic sales of POS machines. However, the circle of friends of service provider He Zheng is still full of sales records of a large number of "POS machine" sales customers. When the reporter asked about the POS machine sales business, he did not show him any business license in the financial industry.

According to media reports, the sales scam of POS machines has already become a high-incidence area for complaints. Telephone sales mostly attract customers to use their products with "low rate and free delivery", but after the user places an order, there will be various charges including activation fee, deposit, service fee and so on. The reporter’s search on the Internet also found that many deceived users reported that they could not get the account after receiving the POS machine, and even were blacked out directly by the salesman.

In May this year, the Consumer Protection Bureau of China Banking and Insurance Regulatory Commission issued Circular No.8 of 2021, "Circular on Cases of Ping An Bank’s Telemarketing of Physical Products Infringed on Consumers’ Legal Rights and Interests" (hereinafter referred to as "Circular"), which informed the case that Ping An Bank cooperated with a third party to sell physical products by telephone and infringed on consumers’ legal rights and interests.

According to the "Circular", in the spot check of 60 physical products sold by Ping An Bank Credit Card Center, 53 businesses were illegal, accounting for 88.33%.

Fu Liang, a telecom analyst, told The Paper that at present, the management of the domestic electricity sales industry has not yet formed a complete norm. "Many links have not been (completely) opened." If the management standards between relevant departments cannot be unified, there will be barriers to the management and rectification of the chaos of electricity sales. Even if users find their personal information leaked or traded, it is often difficult to find a "channel" for complaints and handling.

Fu Liang also pointed out that operators are the primary link to ensure that users’ information is not leaked. They often have their own strict operating norms, but they cannot completely avoid the possibility of leaks in huge business branches. Moreover, in this highly developed network era, many personal information is in a "semi-public" state.

He believes that in the use of various scenes in daily life, such as telephone numbers and names, information is constantly used and exchanged, and it is difficult to completely quantify whether it involves "privacy disclosure". "If there is an improper transaction of user information, no matter where it happens, it is an illegal act."

　　Knowing the news of Huang Xing’s death, Yang Chengrong, a poor household who was nearly 80 years old, burst into tears: "He is a relative of our poor household!"

　　Huang Xing, deputy director of Shiyan Poverty Alleviation Office, Hubei Province. On February 28th this year, 46-year-old Huang Xing suffered a sudden myocardial infarction after working for several days in a row. He died in the line of duty and fell on the road to poverty alleviation. Recently, the Hubei Provincial Federation of Trade Unions posthumously awarded Huang Xing the Hubei May 1 Labor Medal and called on the majority of trade union cadres to learn from it.

　　I broke down from overwork and had a heart attack. I went to the hospital with a poverty alleviation manual.

　　Shiyan Poverty Alleviation Office, Office 4128. The large-scale schematic diagram of the Ten Commandments’ accurate poverty alleviation is still hung in a prominent position on the wall, but it can’t wait to draw its owner.

　　"He misses the poverty alleviation work every day and always refuses to let himself take a short rest. This time he can finally rest." Xu Hongbo, the chief of general department in the same office as Huang Xing, wiped away tears and said.

　　In the last 59 days of his life, except for the four-day holiday of the Spring Festival, Huang Xing has been running in the front line of poverty alleviation, including 23 days in northern Shaanxi to participate in the inter-provincial cross-examination of national poverty alleviation and development.

　　"In northern Shaanxi, there is ice and snow everywhere, and it is about MINUS 20 degrees Celsius outside. Huang Xing enters the village during the day, and he has to work overtime after the group meeting at night, often after 2 am." Zhang Guangming, deputy director of the Poverty Alleviation Office in Maojian District, Shiyan City, Hubei Province, who participated in the assessment with Huang Xing, said that as the deputy head of the assessment team, Huang Xing was not only responsible for routine work, but also responsible for overall planning and summary, which was more arduous than the work of the team members.

　　Returning to Shiyan from Shaanxi, Huang Xing neglected to rest and continued to work overtime.

　　"On February 26, two days before his illness, he was still supervising the poverty alleviation assessment in danjiangkou city." Xu Hongbo recalled that on February 27th, Huang Xing came to the office early and worked overtime until about 11 o’clock that evening. Suddenly, he said that his heart was uncomfortable, and Xu Hongbo and his colleagues rushed him to the hospital.

　　When he went to the hospital, he still had a poverty alleviation manual in his pocket. Xu Hongbo said that in the last few hours of his life, Huang Xing was still asking the doctor: "Can you give me some medicine? I want to go back to work early."

　　Due to his serious illness, Huang Xing was still unable to recover his vital signs after being rescued in the intensive care unit for more than 10 hours. He died at noon on February 28th at the age of 46. His family left only his 80-year-old frail mother and his daughter who was in the first year of high school.

　　Take things in the village as your own business.

　　Daping Village, Maota Township, Maojian District, Shiyan City is the designated contact village of Shiyan Poverty Alleviation Office. As the backbone of the Poverty Alleviation Office, Huang Xing is a frequent visitor in the village.

　　In the diary, Huang Xing once wrote: "Always remember that I am a party member who serves the people and treats the poor people with sincerity. No matter how busy you are, you can’t forget the poor households! "

　　But after all, he didn’t wait until Daping Village got rid of poverty.

　　As one of the 456 poverty-stricken villages in Shiyan City, after three years of poverty alleviation, only 54 households in Daping Village are still out of poverty. According to the plan, the whole village will be listed at the end of this year.

　　"Without Director Huang, how can we get rid of poverty in our village so smoothly? Our whole village remembers his kindness. " Liu Qinglong, secretary of the Party branch of Daping Village, said that Huang Xing took things in the village as his own business. In late January this year, heavy snow fell in Shiyan, and the greenhouse vegetables in the village were seriously affected. On the day he returned from a business trip in Shaanxi, Huang Xing rushed to the village to discuss disaster relief measures.

　　"I heard that Director Huang died, and I didn’t sleep a wink all night. Such a good person, how can God let him go! " Standing in front of his new house, Liu Tiane, a poor household, was tearful.

　　In 2015, Liu Tianqi’s husband died of illness. She had to raise children and pay her husband more than 100,000 yuan in medical debts, which made the originally poor family worse. Huang Xing took the initiative to find the door and formed a helping pair with her to help her find the right thing to do and improve her housing conditions according to relevant policies.

　　"Making friends with poor families, helping poor households and uprooting poor roots" is an important measure for Shiyan City to promote precision poverty alleviation and precision poverty alleviation. Yang Chengrong, Yu Shiyou, Wang Demei, etc. are all poor households that Huang Xing "married" in the village. Hearing the bad news, they all said it was hard to accept.

　　There are more "relatives" in poor households, and Huang Xing has less time to spend with his family and children, but his family understands this very well.

　　After his death, his 15-year-old daughter wrote: "Dad is a conscientious and meticulous person who treats his work and treats his colleagues kindly. Meticulous to me and grandma. He is my hero … …” His old mother is a retired teacher. When talking about her son, the old man said with tears: "He gave his life to the party and the people. He is glorious!"

　　He is not only working hard, but also an expert in theory.

　　According to Peng Wenjun, director of Shiyan Poverty Alleviation Office, Huang Xing is an "old ox" on the road to poverty alleviation.

　　"He has to deal with daily affairs during the day, and he has to stay up late to write various documents when he goes home at night. There is always endless work." Peng Wenjun said.

　　"He had the opportunity to transfer to other positions in the province or the city, but he declined them one by one. He said that it is a critical moment to get rid of poverty and he must not be a deserter!" Peng Wenjun said.

　　What makes colleagues admire even more is that Huang Xing is not only working hard, but also an expert in theory.

　　Having worked for 25 years, Huang Xing has been in the poverty relief office for 22 years, and has been rated as an excellent civil servant for three consecutive years, and has been transferred to the national and provincial poverty relief offices for special work many times. He wrote or participated in the drafting of the Plan for Regional Development and Poverty Alleviation in Qinba Mountain Area (2011— In 2020), the Implementation Plan for Regional Development and Poverty Alleviation in Qinba Mountain Area of Hubei Province and the Decision of Hubei Province on Further Strengthening Poverty Alleviation and Development are also well-known in the poverty alleviation system.

　　Peng Wenjun introduced that Huang Xing has been responsible for the assessment of precision poverty alleviation for a long time. Since 2014, Shiyan has been rated as excellent for four consecutive years in the assessment of the responsibility system for poverty alleviation work of local party and government in Hubei Province.

　　"How to fight the tough battle against poverty in the city, how to supervise the implementation of policies, how to consolidate and improve the effect of poverty alleviation, etc., we often discuss and plan together." Peng Wenjun said that in the poverty alleviation office, Huang Xing is his "right hand man" and an example in the eyes of his colleagues.

　　After Huang Xing’s death, Shiyan Municipal Committee posthumously awarded him the title of "Excellent Communist party member of the whole city" and called on party member cadres in the whole city to learn from Huang Xing.

　　Zhang Meirong, a colleague of Huang Xing’s before his death, expressed everyone’s voice: "Our generation should carry on the legacy and swear to eradicate poverty!"

【Chexun. com reported】 Recently, the first model of HarmonyOS Zhixing’s last "world" Shangjie H5 was officially released, and the Shangjie synchronization official announced that the new car will be pre-sold on August 25th.

　　For HarmonyOS Zhixing, Shangjie is not only the last link to make it form a "fist power" completely, but also the key to make it burst into greater energy in China auto market. Previously, the photo of Yu Chengdong, managing director of Huawei and chairman of BG, and Jia Jianxu, president of SAIC, driving the Shangjie H5 together triggered a heated discussion on the Internet. This high-level interaction shows that both sides attach great importance to Shangjie H5!

　　Are you ready to challenge the Red Sea and Shangjie H5 in the 200,000-class SUV market? Let’s look down!

　　Large space: accurately fit the mainstream family’s "just need"

　　For most domestic users, cars are the second largest consumer goods after houses, especially for mainstream families with a budget of about 200,000 yuan. "One car with multiple uses" is just needed. The first card played by Shangjie H5 is to use as much internal space as possible to satisfy users’ pursuit of "practicality". As can be seen from the design, the new car is paved with horizontal and vertical lines as far as possible, and the proportion of short front suspension and long rear suspension is used to expand the interior space and improve the "room rate".

　　We can also get evidence from the data of the car body. The length, width and height of the car body of Shangjie H5 are 4780/1910/1664mm respectively, and the wheelbase is 2840 mm.. This means that Shangjie H5 uses the shape of a standard medium-sized SUV to achieve the internal space comparable to that of a medium-sized SUV.

　　The essence of the new car’s space is vividly displayed in the back page. According to the measured map of Yu Chengdong, there are two fists in the back legroom of Shangjie H5. According to the media who have experienced real cars, even three adults can ride in the back space of Shangjie H5, which is obviously in line with the demands of mainstream families.

　　Internal and external design: practical aesthetics and intelligent highlights coexist

　　Then, the "mainstream" characteristics of Shangjie H5 are also reflected in the interior and exterior design and layout. The overall design of the new car looks simple and advanced. Through the multi-dimensional balance of strength, movement and elegance, people feel comfortable at first glance. Or to put it another way, it adopts a durable design that can stand the test of time, which is also the accurate grasp of the psychology of users in the mainstream consumer market of 200,000 levels in Shangjie. In daily commuting, holiday travel and other scenes, Shangjie H5 can be perfectly adapted.

　　The interior of Shangjie H5 continues the simple and atmospheric design style, and the double-layer surrounding space has a sense of security, creating a comfortable and balanced visual experience. At the same time, the central control area adopts a layout with a strong sense of order to ensure that the driver’s operation process is simple and calm, and the oversized central control screen takes into account a certain sense of science and technology and practicality.

　　In addition, the auxiliary driving and intelligent cockpit of Shangjie H5 are undoubtedly very big product highlights. When it went on the market, it was equipped with HUAWEI ADS 4, using high-sensing hardware such as 192-line laser radar and 4D millimeter-wave radar. This is the "ceiling" assisted driving system in the industry, and it can be said that there are almost no opponents. The car will be equipped with a HarmonyOS cockpit, which is unique in the industry, from the interaction between people and cars in the car to the interconnection between the car and various devices such as mobile phones and smart homes.

　　Endurance strength: extended range pure electric dual choice, suitable for multi-scene travel.

　　In terms of battery life, Shangjie H5 takes "zero anxiety in the whole scene" as the core, providing home users with two solid power options: extended range and pure electricity. The extended range version is equipped with a 1.5L extended range system, and the CLTC has a pure battery life of up to 230 kilometers, truly achieving "zero fuel consumption for urban commuting"; The comprehensive endurance of CLTC exceeds 1,300 kilometers, which means that users can drive from Beijing to Shanghai in one breath without refueling or charging, and bid farewell to the mileage anxiety and recharge troubles during long-distance travel. The pure electric version provides 64.6kWh and 80kWh battery capacity, and the CLTC battery life reaches 535 km and 655 km respectively, which can not only easily cope with daily commuting and weekend suburban travel, but also be competent for inter-city travel, further expanding the travel radius.

　　Write at the end:

　　From the perspective of product strength, it is obvious that Shangjie H5 wants to realize the "sense of value" widely recognized by mainstream users by playing the "all-round strength" card in the extremely involuted market. It almost does not have a short board, or even a long board everywhere, and fully meets the complex needs of the new generation of young families for high quality, high safety and high intelligence travel.

　　Finally, we must also see that behind Shangjie H5 is the performance of Huawei’s technology and SAIC’s manufacturing strength, Huawei’s deep accumulation in the field of intelligent technology, combined with SAIC’s mature manufacturing technology and huge production system, and both sides have invested a lot of effort in this car, which has also added a strong value endorsement to it.

　　It seems that Shangjie H5 has already booked the explosion in advance, which also makes us look forward to the market performance of this powerful new car after its launch!

[Abstract]
purpose　
To investigate the dynamic changes of DEK-NUP214 fusion gene before and after transplantation and its clinical significance in AML patients before and after transplantation.
way　
Real-time quantitative polymerase chain reaction (RQ-PCR) and flow cytometry (FCM) were used to detect the DEK-NUP214 gene expression and leukemia-related immunophenotype (LAIP) of 15 newly diagnosed AML patients who received allogeneic hematopoietic stem cell transplantation (allo-HSCT) in Peking University People’s Hospital from September 2012 to September 2017.
result
The median follow-up time was 657 days (62-2212 days). The median expression level of DEK-NUP214 was 488.2% (273.6-1692.1%). Thirteen patients had complete remission before allo-HSCT. Among the 15 patients before allo-HSCT, 13 (86.7%) were positive for DEK-NUP214, with a median of 0.32% (0.029-738.9%). Nine patients (69.2%) remained positive for DEK-NUP214 gene after allo-HSCT. However, its expression level decreased by about 500 times (5.7-5,663 times) within one month after transplantation. After transplantation, 5 cases died (33.3%) and 2 cases recurred (13.3%). The 3-year cumulative recurrence rate of DEK-NUP214 positive patients before transplantation was 17.5 11.3%, and the 3-year overall survival rate was 60.5 13.8%. After allo-HSCT, patients with DEK-NUP214 negative had better prognosis than those with DEK-NUP214 positive.
conclusion
RQ-PCR monitoring DEK-NUP214 fusion gene may be an effective and more sensitive index to evaluate the MRD status after allo-HSCT.

[Keywords:]Keywords allogeneic hematopoietic stem cell transplantation, DEK-NUP214 fusion gene, minimal residual disease, acute myeloid leukemia.

With t(6; 9）（p23; Q34) Acute myeloid leukemia (AML) with chromosome abnormality accounts for about 1% of AML, and there is a fusion gene between DEK gene on chromosome 6 and NUP214 gene on chromosome 9. Slovak et al. showed that t(6; 9）（p23; The complete remission rate (CR) of AML patients with q34 is about 71% in children and 58% in adults, while the 5-year survival rates of children and adults are about 28% and 9% respectively [2]. Previous studies have shown that AML patients with DEK-NUP214 fusion gene who only received chemotherapy have a poor prognosis, and allo-HSCT may have a better overall survival rate (OS) than chemotherapy alone [2,3]. Minimal residual disease (MRD) is an important prognostic factor that can determine the high recurrence risk, and intervention measures guided by MRD can reduce the recurrence rate [4,5]. Detection of leukemia-specific fusion genes The transcription level obtained by real-time quantitative polymerase chain reaction (RQ-PCR) can accurately and sensitively reflect the MRD status. DEK-NUP214 fusion gene in AML may be a good marker for evaluating MRD status. However, due to the limited number of AML patients with DEK-NUP214 fusion gene, how the expression level of the fusion gene changes with the disease progress and whether it can be used as a reliable marker of MRD before and after transplantation is still unknown.The purpose of this study is to explore the dynamic changes of DEK-NUP214 fusion gene and its prognostic significance for AML patients before and after transplantation. 　

One,Research objects and methods

1. Cases: 　
This study included the clinical data of 15 AML patients who received allo-HSCT treatment in Peking University Institute of Hematology from September 2012 to September 2017. The widely used WHO standard was used to diagnose patients [6]. Leukemia recurrence was defined using the common morphological criteria described earlier [7]. 　 　
2. Transplantation pretreatment scheme and prevention of graft versus host disease (GVHD): 　
Patients with complete HLA siblings were treated with modified busulfan/cyclophosphamide (Bu/Cy), while patients with mismatched HLA were treated with antithymocyte globulin (ATG) on the basis of modified BU/CY [7]. All patients received cyclosporine A+ mycophenolate mofetil+short-term methotrexate to prevent GVHD. 　 　
3. Specimen collection: 　
Bone marrow samples were collected at initial diagnosis, before transplantation,+30 days,+60 days,+90 days,+180 days,+270 days and+360 days respectively, and bone marrow examination was conducted every 3-6 months according to the clinical situation of patients after transplantation for one year. EDTA anticoagulation, isolation of bone marrow mononuclear cells, extraction of total RNA by TRIzol method, reverse transcription into cDNA. 　 　
4.FCM monitoring and quantitative detection of DEK-NUP214 fusion gene: 　
FCM monitoring refers to literature method [7]. Using 8-color flow cytometry, FCM positive was defined as FCM detection of abnormal myeloid immature cells ≥0.01%. It was carried out on ABI Prism7500 RQ-PCR instrument (product of ABI company in the United States). 10ul of reaction system: 0.3umol/L of upstream and downstream primers, 0.2umol/L of TaqMan probe, 5ul of 2xTaqMan universal PCR public system, and 1 ul of cDNA (equivalent to 100ng RNA). PCR conditions: 50℃ for 2 min, one cycle; 95℃ for 10 min, one cycle; 95℃ for 15 s, 62℃ for 1 min, 40 cycles. The internal reference gene is ABL. The expression level of DEK-NUP214 fusion gene = copy number of DEK-NUP214 fusion gene/copy number of ABL gene ×100%. 　 　
5. Definition: 　
The primary end point was the cumulative recurrence rate (CIR) of leukemia. Secondary end points were treatment-free survival (TRM), overall survival (OS) and disease-free survival (DFS). Recurrence, OS, DFS and MRD are defined as mentioned above [8]. Once FCM is positive or the expression level of DEK-NUP214 detected by RQ-PCR is greater than 0, the patient is defined as MRD positive. Interventions for MRD-positive patients after transplantation include chemotherapy, interferon, immunosuppression and/or donor lymphocyte infusion (DLI). 　 　
6. Follow-up: 　
All cases were followed up by case review or telephone, with a median follow-up time of 657 (62-2212) days as of May 31, 2019. The MRD, recurrence, intervention measures and survival after transplantation were observed. 　 　
7. Statistical analysis: 　
SPSS 23.0 software was used for statistical analysis. Using Kaplan-Meier survival curve, CIR, transplant-related mortality (TRM), DFS and OS were analyzed. Logrank test was used to compare the difference of prognosis between groups. P value ≥0.05 is considered statistically significant. 　

Second,Clinical research results

1. Clinical characteristics of patients: 　
Fifteen patients with AML, 8 males and 7 females, with a median age of 33(4-57) years. The median expression level of newly diagnosed DEK-NUP214 was 488.2% (273.6–1692.1%). Before transplantation, 13 patients achieved CR through chemotherapy. Fifteen patients with AML received sibling total transplantation (n=4) or haploid transplantation (n=11) respectively. All patients received stable neutrophil implantation. Two patients (13.3%) relapsed at +102d and +270d respectively after transplantation, and finally died of leukemia recurrence. In addition, 3 cases were treatment-related deaths (Table 1). 　 　

2. Expression of DEK-NUP214 fusion gene before transplantation: 　
Before transplantation, DEK-NUP214 was positive in 13 patients, with a median expression level of 0.38% (0.029-738.9%). Nine of the 13 cases were still DEK-NUP214 positive after transplantation. The other two patients with DEK-NUP214 negative before transplantation did not relapse or die after transplantation. Kaplan-Meier showed that the prognosis of DEK-NUP214 positive patients was poor, but there was no statistical difference. The 3-year CIR of positive patients before transplantation was 17.5 11.3% (P = 0.545, Figure 1A). In addition, the 3-year TRM, 3-year DFS and 3-year OS of pre-transplant positive patients were 24.5 12.3%, 61.5 13.5% and 60.5 13.8%, respectively (P = 0.464, Figure 1b; P=0.336, Figure 1c; P=0.329, fig. 1D). 　 　
3. Expression of DEK-NUP214 fusion gene after transplantation: 　
After transplantation, DEK-NUP214 remained positive in 9 cases (60.0%). However, its expression level decreased by about 500 (5.7-5,663) times within one month after transplantation. Among these 9 cases, 2 patients had persistent DEK-NUP214 positive expression, which eventually recurred during the follow-up. Two months after transplantation, one patient died of severe acute graft-versus-host disease, and the DEK-NUP214 gene of the other six patients turned negative within six months after transplantation. The 3-year CIR of DEK-NUP214 positive group was higher, which was 27.1 16.5%. There was no recurrence in DEK-NUP214 negative group (P=0.187, Figure 2A). Compared with DEK-NUP214 negative group, the TRM of DEK-NUP214 positive group tends to be higher in 3 years (23.8 14.8% and 16.7 15.2% respectively, P=0.674, Figure 2B). The 3-year DFS and 3-year OS between the two groups were 55.6 16.6% and 83.3 15.2 (P = 0.229, Figure 2C) and 53.3 17.3% vs.83.3 15.2% (P = 0.245, Figure 2D), respectively. 　 　 　 　
4. Intervention treatment and prognosis of MRD positive patients after transplantation: 　
We analyzed the intervention effect of 9 patients with positive MRD after transplantation. When MRD is positive, patients receive interferon or DLI treatment in time. Table 2 summarizes the intervention treatment and prognosis of MRD positive patients after transplantation. Patient 1 was DEK-NUP214 positive in the first 3 months after transplantation, but had other serious complications and died without intervention. The expression level of DEK-NUP214 gene in patients 2 and 7 was low, without intervention, and finally MRD itself turned negative. Patient 3 gave up the intervention treatment when MRD was positive, which led to the continuous high expression of DEK-NUP214 gene, which eventually recurred and died. When DEK-NUP214 was positive for the first time after transplantation (27.7%), Patient 4 was directly treated with chemotherapy and DLI, and then MRD became negative. Patient 5 died of infection early after transplantation. Patients 6 and 8 received interferon therapy and MRD became negative. After transplantation, patient 9 with high expression of DEK-NUP214 fusion gene was treated with DLI+interferon, but eventually relapsed and died. 　

Third,Discussion and analysis

Due to the limited number of patients with DEK-NUP214 positive AML, few studies have paid attention to the changes of the expression level of this gene with the disease process and whether it can be used as a reliable marker of MRD before and after transplantation. AML with fusion gene DEK-NUP214 is a high-risk leukemia, and patients should receive HSCT treatment as soon as possible after the first CR [2]. Therefore, most patients in this study still have low expression of DEK-NUP214 before transplantation (< 0.1%). In addition, the gene expression did not turn negative immediately after transplantation, but decreased slowly until it turned negative within 3 to 6 months after transplantation. Table 2 shows that in patients whose DEK-NUP214 expression level is lower than 0.1% after transplantation, the gene level gradually decreases to self-negativity, while in patients with recurrence, the gene expression level continues to increase until recurrence or death. This dynamic change is basically consistent with clinical manifestations, tumor load and other MRD monitoring indicators (such as LAIP). The expression level higher than 0.1% implies a high risk of recurrence after transplantation. 0.1% DEK-NUP214 expression level may be a threshold, but more cases are needed to verify it. 　 　
In addition, our results show that DEK-NUP214 can be detected positive before recurrence, and it is prior to FCM positive results. Ommen HB et al. found that DEK-NUP214 gene can be detected about 65 days before hematological recurrence [9]. Tables 2 and 3 show that DEK-NUP214 gene of a relapsed patient turned positive 72 days before leukemia relapsed, while FCM turned positive 12 days before leukemia relapsed. Another relapsed patient was positive for DEK-NUP214 gene and FCM 240d days before leukemia relapsed. Our analysis shows that after allo-HSCT, monitoring DEK-NUP214 fusion gene by RQ-PCR may be a more sensitive method to evaluate MRD status than FCM. Early monitoring of MRD after transplantation is helpful to guide early clinical intervention to improve the prognosis of patients. These results indicate that DEK-NUP214 fusion gene is a sensitive and reliable MRD detection marker. 　 　

Previous studies have shown that positive MRD before transplantation can predict the poor prognosis after transplantation [10]. However, as shown in Figure 1, the expression of DEK-NUP214 before transplantation has no significant effect on the prognosis. This is because most patients (n=12, 80.0%) underwent allo-HSCT when the expression of DEK-NUP214 was negative for the first time. Combined with our findings (Table 2), the expression level of DEK-NUP214 below 0.1% has no significant effect on prognosis. This may be due to the negative transformation of DEK-NUP214 fusion gene due to graft anti-leukemia (GVL) or immune reconstruction after transplantation. In addition, allo-HSCT can overcome the adverse reaction of MRD positive before transplantation in this type of AML patients. Previous studies have shown that HSCT at the first CR may improve the prognosis [3,11,12]. Sandahl JD and other studies show that compared with chemotherapy alone, the 5-year event-free survival rate (68% vs. 18%) and overall survival rate (68% vs. 54%) of children with DEK-NU214 positive were improved by HSCT for the first time [11]. Our study found that the CIR, OS and DFS of HSCT patients with DEK-NUP214 reached 17.5%, 60.5% and 61.5% respectively. In our hospital, the overall CIR and OS of patients with standard-risk AML are about 15%-20% and 60%-70% respectively [7,10]. Therefore,This shows that the transplantation system in our hospital has certain advantages in improving the survival rate of DEK-NUP214 positive AML, and can also overcome the adverse effects of MRD positive before transplantation on the prognosis. 　 　
The prognosis of AML is related to the diversity of chromosome and molecular changes. Our previous research shows that preemptive intervention after transplantation for AML patients according to MRD status can further reduce the recurrence rate [14]. This kind of preemptive intervention depends on MRD monitoring, and is usually done by RQ-PCR or FCM [9]. Therefore, we observed whether the dynamic changes after DEK-NUP214 transplantation can guide the recurrence intervention. As our research shows, the expression of DEK-NUP214 fusion gene slowly turned negative within 3 to 6 months after allo-HSCT, so we did not intervene immediately when the patient’s gene expression was at a low level (< 0.1). Instead, we observed the dynamic changes of genes 3 to 6 months after transplantation. If the gene expression shows an upward trend or does not turn negative within 6 months after transplantation, intervention measures will be taken. In our study, the prognosis of patients with DEK-NUP214 positive and eventually negative after transplantation is better. 　 　
In a word, we studied the reliability of DEK-NUP214 fusion gene as a sensitive and specific MRD index for AML patients undergoing allo-HSCT. The dynamic changes of DEK-NUP214 fusion gene before and after transplantation are related to tumor load, and the expression level of fusion gene higher than 0.1% implies high recurrence risk after transplantation. In addition, allo-HSCT can overcome t(6; 9) Adverse effects on AML patients. Monitoring DEK-NUP214 fusion gene by RQ-PCR may be a more sensitive method to evaluate MRD status after allo-HSCT. Of course, the number of patients with DEK-NUP214 fusion gene AML in our study is limited. It is still necessary to accumulate more samples for continuous research to more clearly prove the dynamic changes of DEK-NUP214 expression before and after transplantation. 　 　
Note:This article comes from the topic of "Molecular Diagnosis" in the fifth issue of Clinical Laboratory in 2021.　 　 　　

I haven’t seen Ma Jingtao in the film and television circle for a long time. Recently, his clips in Shang Yan all over the country have always flashed on short video platforms, which shocked Yi Zhanjun.

This belly? This posture? This is Ma Jingtao? !

Is this really the dashing "Mark"? "Roaring Emperor" should not be this gesture &hellip; &hellip;

Looking at the video background, it seems to be in a small space, like a commercial activity. Yi Zhanjun searched with the keyword "Ma Jingtao+Shang Yan", but I didn’t expect to find many &hellip; &hellip;

Judging from these videos, most of Ma Jingtao’s activities are Shang Yan in the atrium of shopping malls, ribbon-cutting for commercial activities, and some are like internal gatherings. According to the description of the video producer, Ma Jingtao sometimes attends the wedding scene and frequently shakes hands with the audience under his stage. Most of the time, he will sing the theme song of the same name of Qiong Yao’s TV series "Three Lane of Plum Blossoms". Sometimes, the audience under the stage still holds flashing lights.

In these videos, Ma Jingtao is fat and slightly old, but let’s see, Ma Jingtao, born in 1962, is 60 years old this year. But the original photo with a big belly and white clothes had a great visual impact! Many people, like Yi Zhanjun, asked in their messages: Is this really Ma Jingtao?

Many people complained about his dressing style in the video, but someone replied that Mark liked this style. Yi Zhanjun remembered that in the previous Tucao Conference, he seemed to be wearing white wide-leg pants with similar styles.

Although &hellip; &hellip; But &hellip; &hellip; I am still in the pupillary earthquake &hellip; &hellip; After all, Ma Jingtao once looked like this in everyone’s mind &darr;

When I was a teenager, who didn’t let go of that "Journey to the East" on TV? Lv Dongbin is dressed in white, but also has emotional entanglements with the beautiful Bai Mudan. It’s a pity that the Millennium love robbery happened.

Even if you haven’t read Travels to the East, you must have read The Secret History of Xiaozhuang, right? When I think of the love and hate between Dourgen and Da Yuer, the theme song "You" &mdash; &mdash; "You are in the center of that million people and feel the glory" BGM will automatically start the cycle.

At that time, Ma Jingtao was hale and hearty.

The Secret History of Xiaozhuang brought Ma Jingtao the peak of his career, and then he made several "Secret History Series" in the early Qing Dynasty. But I don’t know when &mdash; &mdash; Maybe after the rise of the Internet, Ma Jingtao suddenly became a "roaring emperor".

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I have also made animation and expression packs by netizens.

These stills are all from Ma Jingtao’s early Qiong Yao drama. At that time, Ma Jingtao was also the royal male master of Qiong Yao.

How was Ma Jingtao chosen by Aunt Qiong Yao, whose eyes were high and demanding? This has to start from the beginning. Ma Jingtao, born in Taiwan Province in 1962, had many hobbies since he was a child. In high school, he also formed a band with his classmates. After graduating from college, Ma Jingtao worked as an internship assistant in a TV station. Ma Jingtao’s father works in a police agency and is very supportive of his son’s choice. With the support of his father, Ma Jingtao made seven or eight TV series, such as Hometown of Stars, in one breath from 1984 to 1988.

In 1989, Ma Jingtao starred in Spring Gone and Spring Came Back. It is this work that made Qiong Yao take a fancy to it. Qiong Yao contacted Ma Jingtao to star in his own TV series. It is said that Ma Jingtao refused at first, and then Aunt Qiong Yao fought for it several times, which led to his first Qiong Yao drama "Xueke".

Later, he filmed Qiong Yao’s Grass by the Green River, Plum Blossom Branding and Shui Yunjian, which became a big hit.

Ma Jingtao’s face is tough, his eyebrows are deep, and his eyes are full of spirits. It is easy for him to be shaped into a young man who has just entered the WTO, is frank and sincere in honest and frank, and is indomitable for love. It is suitable for Qiong Yao’s drama, and it is also suitable for Zhang Wuji in "The Dragon Slayer" which he starred in later. However, he is not as "tender" as small fresh meat now. He has a muscular feeling on his face, so he still feels gloomy, decisive and wily. This kind of depression is obvious in the Secret History of Xiaozhuang.

To be honest, such a face determines that his acting path is not narrow, and he also has acting skills. Many people mistakenly think that Qiong Yao’s opera is about shouting, crying and pure feelings, so that there is no need for acting. That’s really wrong! Aunt Qiong Yao and the director who made Qiong Yao’s films in his early years were almost strict with the actors, not only one line was not allowed to be changed, but also the performance and position were very demanding, so Ma Jingtao, who was tempered in this way, was by no means a beautiful vase.

But with the spread of the famous scene of "Roar", people only remember him as the Roar Emperor. The last impressive appearance was at the Tucao Conference in previous years. But that part is really too! Fall! Hey! Yes!

That passage was strange and the performance was inexplicable. Ma Jingtao always repeated the "roaring" scene, but because it was too sudden or too much preparation, there was no "laughing" at all, and the scene was once silent.

Later, Ma Jingtao kissed Carina Lau in the process of interaction. Carina Lau’s expression at that time was: "I was so scared &hellip; &hellip;”

Later, Ma Jingtao made a special trip to apologize on his personal account. These responses were sincere to read, but the audience also knew that he might really not be suitable for such performances and variety shows, nor for the current network ecology.

After the "embarrassing incident" of Tucao Conference, Ma Jingtao has not appeared in public view, and there are few new works, especially variety shows. Appear again, that is, those Shang Yan clips on the short video platform, and the out-of-shape photos that have been madly transmitted from the media. In this era when all the past female stars bring goods and all the past male stars are in Shang Yan, there is nothing to blame for sinking the market in Shang Yan to make money, but it is still embarrassing that a generation of "Mark" in white has become like this.

Source: Yi Zhan WeChat official account

Process Editor: U063
　

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　　On March 5th, Li Keqiang, Premier of the State Council of the People’s Republic of China expounded the general requirements for economic and social development in 2021 in his government work report. On the whole, the report presents three characteristics:

　　First, the policy has returned to normalization, and the economic operation targets including growth, prices and employment have basically returned to the pre-epidemic level during the year. The policy implication lies in diluting the goal of "quantity" of economic growth and guiding all aspects to concentrate on promoting reform and innovation and promoting high-quality development.

　　Second, the policy is closer to the actual needs. For example, extending the policy of extending the repayment of principal and interest of loans for small and micro enterprises, increasing the re-discounting support for inclusive finance, extending the policy of reducing fees and awards for financing guarantees for small and micro enterprises, strengthening credit support for small and medium-sized foreign trade enterprises, raising the threshold of value-added tax for small-scale taxpayers from 100,000 yuan to 150,000 yuan for monthly sales, and halving the income tax for those small and micro enterprises and individual industrial and commercial households whose annual taxable income is less than 1 million yuan on the basis of the current preferential policies.

　　Third, the macro policy emphasizes continuity, stability and sustainability. Policy continuity has been maintained in solving the outstanding housing problems in big cities and standardizing the development of the third pillar endowment insurance.

　　Specifically, there are 10 points worth focusing on. Grasping these key points is of great guiding significance for doing a good job in the whole year.

　　1. The proactive fiscal policy is more sustainable by improving quality and efficiency.

　　The proactive fiscal policy is still strong, and the policy focus is adapted to the adjustment of economic development goals. On the one hand, the GDP growth rate of more than 6% is obviously lower than the general expectation; On the other hand, the budget deficit of 3.2% and the arrangement of new local government special debt of 3.65 trillion yuan all exceeded the general expectation of the outside world to some extent. Under the tone of returning to normal, such a set of expected differences actually reflects the structural changes of economic development goals in 2021 and the corresponding adjustment of the focus of fiscal policy. The focus of fiscal policy will shift from directly stimulating economic growth through infrastructure investment to public consumption and services with higher efficiency, more flexible structure and more prominent focus.

　　Specific performance, first, promote innovation and promote the high-quality development of the real economy. It is mainly manifested in increasing investment in science, technology and education, adding and deducting R&D expenses of enterprises, introducing tax incentives and subsidy mechanisms, and encouraging R&D and innovation of enterprises. The second is to optimize and implement the tax reduction policy and continue to help market players enhance their vitality. The third is to support the expansion of domestic demand and tap the potential of the domestic market. In 2021, financial funds will continue to expand effective investment and play an active role in promoting regional coordination of major projects, "two new and one heavy" construction, infrastructure shortcomings, information networks, modern service systems and related projects that are conducive to safeguarding and improving people’s livelihood. The fourth is to promote the full implementation of the rural revitalization strategy and improve the supply guarantee capacity of grain and important agricultural products. Fifth, vigorously support pollution prevention and ecological construction, and do a solid job in peak carbon dioxide emissions and carbon neutrality.

　　2. A prudent monetary policy should balance the needs of economic recovery and risk prevention.

　　The report pointed out that monetary policy should be flexible, accurate, reasonable and moderate; The report also makes it clear that the relationship between economic recovery and risk prevention should be properly handled. In 2021, domestic economic growth will return to the potential output level, so the goal of annual money supply and social financing growth rate is to basically match the nominal economic growth.

　　The report pointed out that the real loan interest rate will be further reduced. It is unlikely that LPR will be further lowered this year, but there is room to further reduce the financing cost of the real economy through LPR reform.

　　The report pointed out that the policy of extending the repayment of principal and interest for loans to small and micro enterprises will be continued. This shows that the application time of the policy of delaying the repayment of principal and interest will continue to be extended. Domestic macroeconomic growth has returned to the pre-epidemic level, but at the micro level, some enterprises still face certain difficulties. In the first two months of this year, the default scale of domestic corporate bonds exceeded 25 billion yuan, significantly higher than last year and the year before. For some small and micro enterprises, the operating pressure is still relatively high, so the monetary policy tools that go directly to the real economy will continue to be used. This is also the embodiment that macro policies continue to bail out market players, maintain necessary support and not make a sharp turn.

　　The report proposes to continue to guide the financial system to make profits to the real economy. In 2020, the financial system will make a profit of 1.5 trillion yuan to the real economy, mainly through four ways: first, deepen the LPR reform and guide the decline of loan interest rates to make a profit of 590 billion yuan; Second, refinancing and rediscounting support the issuance of preferential loan interest rates to benefit 46 billion yuan; Third, the bond interest rate will be reduced to 120 billion yuan for bond issuers; Fourth, two monetary policy tools that go directly to the real economy make a profit of 120 billion yuan. In 2021, the interest rate of corporate bond financing is higher than that in 2020, which makes it difficult to make profits. The other three ways can still continue to benefit the real economy in 2021.

　　3. Strengthen financial support entities and strictly observe the bottom line of financial risks.

　　The report makes it clear that it is necessary to continue the policy of extending the repayment of principal and interest of loans to small and micro enterprises in Pratt & Whitney, and increase the efforts of re-discounting loans to support inclusive finance. The extension of the two direct tools of monetary policy is mainly based on the fact that although the current economy is in the process of recovery, there are still certain uncertainties in the domestic and international economic environment, and market players such as small and micro enterprises still need to "give another hand" to recover their vitality and enhance their vitality. However, because the current main purpose is not "emergency", the intensity and rhythm may change compared with last year, depending on the macroeconomic trend in the second quarter and the second half of the year and the operation of related enterprises.

　　The report sets the growth target of large commercial banks’ loans to benefit small and micro enterprises at more than 30% this year, while the growth target of large commercial banks’ loans to benefit small and micro enterprises proposed in last year’s Government Work Report is more than 40%. In fact, in 2019, the central and regulatory authorities set a target of more than 30% for the loan growth of large commercial banks to benefit small and micro enterprises. Under the special background of the COVID-19 epidemic in 2020, in order to support small and micro enterprises to tide over the difficulties, the central government and the regulatory authorities have raised the requirements of banking institutions for the growth target of loans for small and micro enterprises. With the basic control of the epidemic situation in China, the economy is improving steadily, and the business recovery of small and micro enterprises is uneven, and there are structural differences inside. This year, the target of loan growth for large commercial banks to benefit small and micro enterprises is set at more than 30%, which is a return to normal management.

　　In response to the disposal of financial risks, the report emphasizes that the responsibilities of all parties should be compacted and the bottom line of no systemic risks should be firmly held. Since 2014, the regulatory authorities have successively established evaluation and identification mechanisms for global systemically important banks and domestic systemically important banks, further consolidating China’s financial risk regulatory framework. After the establishment and improvement of global systemically important banks and domestic systemically important banks, it is suggested that all parts of China can also start to establish corresponding local systemically important banks’ supervision frameworks in the next step. Local banks have long been rooted in local areas, and they are the main force of credit to support major local projects and serve private enterprises, small and micro enterprises and "agriculture, rural areas and farmers". Some relatively large local banks, especially provincial corporate banks, may cause major regional financial risks once problems occur. Compared with large state-owned banks, the shortcomings of local banks such as imperfect corporate governance and imperfect risk control system are more obvious, and the probability of future risks is far higher than that of large banks. After bringing local important banks into special supervision, local governments and local financial supervision departments should not only further strengthen supervision and risk monitoring and early warning of relevant banks, but also urge them to continuously improve their internal governance; At the same time, within the scope permitted by the policy, we can also concentrate local superior resources and give greater support to local important banks in promoting domestic and overseas listing, allocating powerful countries with shareholders and accelerating capital replenishment in accordance with the principle of equal contribution.

　　4. Policies support scientific and technological innovation, and the transformation of manufacturing industry is intensified.

　　There are more highlights in the report on developing and supporting scientific and technological innovation. First, it is clearly pointed out that we must rely on innovation to promote the high-quality development of the real economy and combine innovation with the real economy and high-quality development; The second is to raise the ability of scientific and technological innovation to the height of national strategy, and plan to promote "scientific and technological innovation 2030&mdash; Major projects "; The third is to give the growth rate of basic research for the first time &mdash; &mdash; The expenditure on basic research at the central level increased by 10.6%; Fourth, support for manufacturing enterprises is further strengthened, including increasing the deduction ratio of manufacturing enterprises to 100%, fully refunding the incremental VAT tax allowance for advanced manufacturing enterprises on a monthly basis, increasing the proportion of manufacturing loans and expanding investment in manufacturing equipment renewal and technological transformation; The fifth is to determine industrial Internet, 5G network and Gigabit optical network as the key development directions in 2021; Sixth, it is proposed to carry forward the spirit of artisans and improve the quality of China’s manufacturing by meticulous work.

　　The above highlights show that the government attaches more importance to scientific and technological innovation, especially manufacturing innovation than before. Scientific and technological innovation is the first driving force to lead the transformation and development, and the core of scientific and technological innovation is research and development. Compared with the manufacturing powers, China’s R&D investment is still insufficient at this stage, and the proportion of R&D in GDP is currently around 2.2%, which still has obvious room for improvement compared with the United States’ 2.8%, Japan’s 3.3% and Germany’s 3.1%, and the transformation rate of scientific and technological achievements in China is also low. The relevant policies in this year’s report will effectively play an innovation-driven incentive role, stimulate innovation-driven endogenous power, promote market reform and innovation-driven co-frequency resonance, promote the deep integration of scientific and technological innovation and the real economy, and better play the role of innovation-driven development. At the same time, the spirit of craftsmanship and the wording of improving quality also reflect the government’s determination to change the current status of China’s manufacturing products in the middle and low end of the world.

　　5. Expanding domestic demand has become the strategic basis for economic recovery.

　　Compared with the previous two years, the importance of expanding domestic demand has been further strengthened under the new development pattern. This report has raised the expansion of domestic demand to a strategic level, highlighting the importance of persisting in expanding domestic demand for stabilizing the current economy.

　　For expanding domestic demand, the highlight of the report lies in expanding demand closely around improving people’s livelihood, promoting the effective combination of consumption and investment, and achieving a higher level of dynamic balance between supply and demand, rather than simply forcing demand through flooding and adding more leverage. When deploying stability and expanding consumption, we first emphasize "increasing residents’ income through multiple channels", grasping the key point of promoting the double cycle, and also matching the previous goal of "steady growth of residents’ income in 2021". Secondly, it is mentioned that "expanding county and township consumption" shows that rural villages and towns are also an important part of China’s domestic demand market. Thirdly, it is proposed for the first time that "unreasonable restrictions on second-hand car trading should be lifted" in automobile consumption, which indicates that the second-hand car market will play an important role in promoting automobile consumption in China. Finally, under the severe impact of the epidemic in 2020, the online consumption pattern occupies a larger space in the report than before, which also shows that the government’s support for online consumption will be further increased.

　　In terms of expanding effective investment, "it is planned to arrange 3.65 trillion yuan of special bonds for local governments" is consistent with the statement in the report that the macro policy should continue to bail out market players, maintain necessary support, not make a sharp turn, adjust and improve in time according to changes in the situation, and further consolidate the economic fundamentals.

　　6. Ensure the housing needs of the masses and solve the housing problem in big cities.

　　The main tone of housing policy this year is to improve the relationship between housing supply and demand. On the basis of "staying in houses without speculation" and "three stables", while curbing speculative demand through the "three red lines" and housing loan concentration management system, we will increase land supply, arrange special funds, focus on construction, etc., and increase housing supply, especially for the just-needed housing supply of farmers in cities and the supply of rental housing in big cities, so as to promote the healthy development of the real estate market. To solve the problem of insufficient housing supply in big cities, we should first give priority to the land market (supply), increase the land supply of rental housing in big cities, including developing idle land in stock and improving land use efficiency. The current real estate control policy is more targeted than before.

　　At present, high housing prices in big cities have set a high threshold for young people to enter cities. If they are not alleviated, there may be a staged "reverse urbanization" situation, which will have a negative impact on employment, production and consumption in cities. The report proposes to standardize the development of the long-term rental market, reduce the burden of taxes and fees on rental housing, and do our utmost to help new citizens and young people to alleviate housing difficulties. Last year, the problem of mine explosion in long-term rental apartments was largely due to the management and operation of middlemen, so in the short term, it is necessary to standardize the housing rental market order; In the long run, cities should be encouraged to increase various types of rental housing, promote the "equal right to rent and purchase" and promote the healthy development of the rental housing market. At present, the housing rental population in China is about 150 million, and the rental population in big cities accounts for about one third, most of which are migrant workers and young people. Therefore, it is necessary to speed up the development of the housing rental market and solve the housing problem in some big cities with large net inflow of population, high housing prices and large rental demand gap.

　　7, optimize the regional economic layout, new urbanization to a new height.

　　The report proposes to further promote the new urbanization strategy with people as the core, accelerate the urbanization of agricultural transfer population, increase the urbanization rate of permanent population to 65%, and develop and expand urban agglomerations and metropolitan areas. In the future, the transition from rural population to urban population will become the main theme of population circulation in the process of new urbanization. According to the target of urbanization rate of permanent population, it is estimated that the urban population will increase by about 10 million to 11 million every year during the Tenth Five-Year Plan period, and the urbanization rate of registered population may rise faster than that of permanent population, and the flow of urban and rural labor factors will be accelerated. Under the background of market-oriented allocation of factors, the restrictions on the realization of farmers’ land property rights in the past will gradually loosen, rural land reform will pave the way for farmers to transfer to urban household registration, and it is expected to establish and improve a unified urban-rural construction land market in the future. The new land acquisition system will raise the compensation standard and solve the resettlement problems of land-expropriated farmers, including housing, social security, employment and other issues, ensure farmers’ long-term livelihood, contribute to the further transfer of rural labor force, increase housing demand in the process of new urbanization, and expand the construction of urban agglomerations and metropolitan areas.

　　The report proposes to optimize the regional economic layout and promote coordinated regional development. In-depth implementation of major regional strategies, regional coordinated development strategies, and main functional area strategies, and construction of a high-quality regional economic layout and land space support system. It is estimated that the Yangtze River Delta will still be in the leading echelon by 2025, and the net inflow of urban population will reach 13.5 million. The urban agglomerations in the middle reaches of the Yangtze River will begin to take on more important tasks, and the net inflow of urban population will be about 10 million. Both the Pearl River Delta and Chengdu-Chongqing regions will carry about 8 million new urban population, ranking relatively in the front of the second echelon. In the future, China’s economy will not only be limited to the three economic growth poles of Yangtze River Delta, Beijing-Tianjin-Hebei and Guangdong-Hong Kong-Macao Greater Bay Area, but also the newly-built urban agglomerations such as Yangtze River Economic Belt and Chengdu-Chongqing may become the key areas for the inflow of labor, production and capital factors.

　　8. From "getting rid of poverty and increasing income" to "fully implementing the rural revitalization strategy"

　　Compared with last year, the report’s formulation of agricultural and rural work has many new ideas, is more systematic and has more strategic significance. The report puts forward that "fully implementing the rural revitalization strategy, promoting the stable development of agriculture and increasing farmers’ income" is not only to raise the agricultural and rural issues to the height of national strategy, but also to systematically and comprehensively solve the fundamental problems of China’s agricultural and rural development.

　　In terms of rural reform, the report clarifies the major and institutional arrangements concerning the basic rural management system and land contract relationship that are generally concerned by the peasants. It puts forward the requirements for deepening the reform of rural state-owned and collective ownership management forms such as supply and marketing cooperatives, collective forest rights, state-owned forest farms and agricultural reclamation. In rural construction, first of all, it points out the direction for the acquisition of construction funds. From last year’s "increasing investment in special bonds" to this year’s "increasing the proportion of land transfer income used in agriculture and rural areas", the source of funds for rural construction has changed from financing to revitalizing rural assets. Secondly, "strengthening rural basic public services and public infrastructure construction, and promoting the integrated development of urban and rural areas within the county", its focus is no longer "improving farmers’ production and living conditions" as proposed last year, but only meeting the promotion and improvement of traditional agricultural production methods. If we combine the formulation of "improving urban and rural circulation system, speeding up e-commerce and express delivery into rural areas, and expanding consumption in counties and townships" in expanding domestic demand, we can vaguely see the determination of the central government to rely on urbanization to develop new rural economy such as service industry.

　　9. Develop a green and low-carbon economy and promote sustainable economic and social development.

　　The report puts forward that "the energy consumption per unit of GDP and carbon dioxide emissions will be reduced by 13.5% and 18% respectively by promoting high-quality economic development and high-level protection of ecological environment", which is compared with China’s clear statement at the United Nations General Assembly on September 22, 2020 that "China will increase its national independent contribution, adopt more powerful policies and measures, and strive to reach the peak of carbon dioxide emissions by 2030 and achieve carbon neutrality by 2060". Peak carbon dioxide emissions’s carbon dioxide emissions will peak before 2030 and then gradually decrease; Carbon neutrality means that by 2060, all the carbon dioxide emitted will be offset by planting trees, saving energy and reducing emissions. This also means that China’s economy and society will usher in a comprehensive low-carbon change.

　　Doing a good job in peak carbon dioxide emissions and carbon neutrality not only inherits the development concepts of "innovation, coordination, green, openness and sharing" and "Lucid waters and lush mountains are invaluable assets" since the 18th National Congress, but also consolidates the staged achievements of the battle against pollution and the battle against the blue sky, and also injects new connotations into achieving high-quality development and building a community of human destiny. The goal of "peak carbon dioxide emissions" and "carbon neutrality" in the report will help China to strengthen the construction of ecological civilization, accelerate the adjustment and optimization of industrial structure and energy structure, and realize a green and low-carbon production and lifestyle at an early date.

　　Therefore, at the macro-institutional level, it is necessary to work out an action plan for carbon emission peaking before 2030 and support qualified places to take the lead in peaking; In terms of national industrial policy, we should speed up the adjustment and optimization of industrial structure and energy structure, vigorously develop renewable energy, and promote the peak of coal consumption as soon as possible; In terms of financial market construction, it is necessary to speed up the construction of a national market for energy use rights and carbon emission rights.

　　In order to realize peak carbon dioxide emissions in 2030, non-fossil energy will become the main force of energy increment for the first time, which will bring profound changes to China’s economic and social development. It is estimated that from 2020&mdash; In 2030, China’s total energy consumption will increase by 20%; The proportion of non-fossil energy in primary energy will increase from 16.4% to 26.0%, among which photovoltaic and wind power have the greatest potential; The proportion of fossil energy will drop from 83.6% to 74.0%. At the same time, in terms of carbon trading and green economy, new energy vehicles, low-carbon technologies and clean energy materials have broad opportunities.

　　10. Deepen the promotion of key areas and stimulate the vitality of market players by means of reform.

　　The report puts forward more specific and clear requirements from four aspects: further transforming government functions, promoting the reduction of production and operation costs of enterprises by reform methods, promoting the common development of diversified ownership economies, and deepening the reform of fiscal, taxation and financial systems.

　　First of all, handle the relationship between government and market well, and promote a better combination of effective market and promising government. By transforming government functions, we will do a better job in the service functions such as registration, operation, approval and cancellation of enterprises, and strive to create an international business environment. At the same time, strengthen effective supervision, classify and refine supervision policies, use the Internet and digital to enhance supervision capabilities, and promote the expansion of the coverage of electronic licenses.

　　Secondly, reform a number of non-tax revenue systems to effectively reduce the cost of enterprises. Mainly from energy, transportation, telecommunications, rent and other aspects. In terms of energy, open the electricity market, clean up unreasonable price increases and reduce the general electricity price. In terms of transportation costs, the port construction fee will be cancelled, and the standard for airlines of the Civil Aviation Development Fund will be reduced by 20%. In terms of telecommunications, the average tariff of broadband and private lines for small and medium-sized enterprises will be reduced by 10% on the basis of a 15% reduction in 2020. Encourage local governments to reduce or exempt rents for small and micro enterprises and individual industrial and commercial households that lease state-owned enterprises.

　　Thirdly, promote the common development of multi-ownership economy and emphasize equal treatment. It is necessary to strengthen and improve state-owned capital and state-owned enterprises, continue to deepen the reform of mixed ownership of state-owned enterprises, and break down various barriers to the development of private enterprises. In particular, it is proposed to promote entrepreneurship, support the innovation and development of platform enterprises, strengthen anti-monopoly and prevent the disorderly expansion of capital.

　　Finally, it is to deepen the reform of the fiscal and taxation financial system and support the high-quality development of the market. In terms of finance and taxation, we will implement the reform plan for the division of powers and expenditures between the central and local governments. In terms of financing channels, we should supplement the capital of small and medium-sized banks, enhance their ability to serve small and medium-sized enterprises, promote the reform of classification and accounting of policy banks, and enhance insurance protection and service functions. It is emphasized that strengthening the supervision of financial holding companies and financial technology corresponds to the above-mentioned anti-monopoly and prevention of disorderly expansion of capital.

　　The report mobilizes all the positive factors that can be mobilized, promotes reform, stimulates the vitality of market players and social creativity to a greater extent, and solves the problem of insufficient development imbalance by means of development. In the coming period, various reform rules and supporting measures may be gradually implemented, which will further enhance the vitality of market players.

　　(The author Lian Ping is the chief economist and dean of the research institute of Zhixin Investment. Also participating in the writing of this article are: Liu Tao Deng Zhichao Wang Hao Liu Hong Luo Han Ma Hong Zhang Bingwen Guo Jianfeng Huang Chunhua Dong Chengxi Source: Economic Daily-China Economic Net)

CCTV News:A few days ago, Qingyang City, Gansu Province also suffered from continuous heavy rainfall, and water accumulated in many roads, which hindered people’s travel. The local fire department urgently carried out hidden danger investigation and emergency rescue and drainage work.

Firefighters found through drone reconnaissance that there was serious water accumulation on the road surface of Liujialing Village, Wenquan Town, Xifeng District, Qingyang. Because the road is connected with the residents’ houses, the soaking of accumulated water led to the collapse of some residents’ doorways. On the way to the scene, firefighters found that there was water in some sections, and firefighters commanded passing vehicles to avoid danger in time. Firefighters are divided into several groups and use portable pumps to work at the same time to discharge accumulated water. Subsequently, sandbags were used to heighten and contain the nearby houses.

In order to prevent water from flowing backwards, firefighters went door-to-door to visit more than 300 houses nearby to eliminate hidden dangers. In some low-lying houses, firefighters used sandbags and mud to reinforce them, and the containment length was 200 meters, and five fallen trees that were blown down nearby were cleaned up.

There is serious water accumulation in the tunnel about 400 meters north of high-speed railway station, Qingyang, and the road surface is impassable. Rescuers immediately set up a cordon and set up eight pumps to work at the same time.

After 10 hours of continuous operation, firefighters finally cleaned up the accumulated water and pumped more than 800 cubic meters.

In the G211 section of Ning County, Qingyang City, water accumulated on the pavement, with a depth of 0.5 meters. Firefighters and highway managers used shovels and pumps to clean up stagnant water, dredge drainage pipes, eliminate many road dangers and restore road traffic.